灵芝多糖真空耦合超声提取工艺优化及其抗帕金森病活性研究-学报杂志社

期刊信息

合肥工业大学（自然科学版），2026年8月，第49卷第8期：1135-1140

DOI: 10.3969/j.issn.1003-5060.2026.08.019

作者信息

朱蔓蕾 $ ^{1} $，李强明 $ ^{1} $，吴淑珍 $ ^{1} $，王成 $ ^{2} $，彭灿 $ ^{3} $，罗建平 $ ^{1} $

（1. 合肥工业大学食品与生物工程学院，安徽合肥 230601；2. 霍山县天下泽雨生物科技发展有限公司，安徽六安 237200；3. 安徽中医药大学药学院，安徽合肥 230012）

摘要和关键词

摘要: 为了提高灵芝多糖的提取率,并评价其抗帕金森病(Parkinson's disease, PD)活性,文章通过单因素实验和正交试验优化灵芝多糖的真空耦合超声提取工艺,并采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的PD小鼠模型评价灵芝多糖的抗PD活性。结果表明:灵芝多糖真空耦合超声提取的最佳工艺条件为超声时间120 min、超声温度50 ℃、超声功率300 W、料液比1:30;在该条件下,灵芝多糖提取率为2.16%,与超声辅助提取法相比,灵芝多糖提取率提高了52.11%。体内抗PD活性实验结果显示,灵芝多糖可显著改善PD小鼠的运动功能障碍,并可抑制多巴胺能神经元变性死亡。研究结果表明,真空耦合超声提取法能显著提高灵芝多糖的提取率,且灵芝多糖具有显著的抗PD活性。

关键词: 灵芝；多糖；真空耦合超声提取法；抗帕金森病(PD)活性；工艺优化

Authors

ZHU Manlei $ ^{1} $, LI Qiangming $ ^{1} $, WU Shuzhen $ ^{1} $, WANG Cheng $ ^{2} $, PENG Can $ ^{3} $, LUO Jianping $ ^{1} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Huoshan County Tianxia Zeyu Biotechnology Development Co., Ltd., Lu'an 237200, China; 3. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China)

Abstract and Keywords

Abstract: To enhance the extraction yield of Ganoderma lucidum polysaccharides, and to evaluate their anti-Parkinson's disease(PD) activity, vacuum-coupled ultrasonic extraction conditions of G. lucidum polysaccharides were optimized by single factor test and orthogonal test, and the anti-PD activity of G. lucidum polysaccharides was evaluated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) induced PD mouse model. The optimal extraction conditions were as follows: ultrasonic time of 120 min, ultrasonic temperature of 50 $ {}^{\circ} $C, ultrasonic power of 300 W, and solid-liquid ratio of 1:30. Under these conditions, the extraction yield of G. lucidum polysaccharides was 2.16%. Compared with ultrasound-assisted extraction, the extraction yield of G. lucidum polysaccharides was enhanced by 52.11% via vacuum-coupled ultrasonic extraction. The in vivo anti-PD activity evaluations demonstrated that G. lucidum polysaccharides could significantly improve the motor dysfunction and inhibit the dopaminergic neuron loss of PD mice. These results prove that vacuum-coupled ultrasonic extraction can be used to enhance the extraction yield of G. lucidum polysaccharides, and G. lucidum polysaccharides exhibit significant anti-PD activity.

Keywords: Ganoderma lucidum; polysaccharides; vacuum-coupled ultrasonic extraction; anti-Parkinson's disease (PD) activity; process optimization

基金信息

安徽省科技重大专项资助项目（201903a07020020）

合肥工业大学学报(自然科学版)

导航菜单

灵芝多糖真空耦合超声提取工艺优化及其抗帕金森病活性研究