Abstract: The homeostasis of trace metal element zinc is closely related to the occurrence and development of Parkinson's disease(PD). The yeast ZAP1 transcription factor has been identified to perceive cellular zinc status and increase the expression of its target gene in response to zinc deficiency. However, there have been few reports on the relationship between ZAP1 and the occurrence and development of PD. In order to better study the function of ZAP1, this paper amplified the full length of the ZAP1 gene using polymerase chain reaction(PCR) and connected it to pUAST to construct pUAST-ZAP1. Drosophila eggs were introduced using microinjection technology to obtain UAS-ZAP1 transgenic Drosophila. The successful construction of the transgenic Drosophila was verified through PCR, and genetic and behavioral methods were used to specifically express the ZAP1 gene in dopaminergic neurons of the transgenic Drosophila. Overexpression of ZAP1 was found to rescue the reduced climbing and jumping ability induced by rotenone in Drosophila. These findings lay a foundation for further studying the function of ZAP1 gene in rotenone-induced PD.
Keywords: ZAP1 gene; zinc; Drosophila; Parkinson's disease(PD); rotenone
合肥工业大学学报自科版