TaqMan probe-based quantitative real-time PCR for detection of adulterants in vermicelli

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Jul. 2026, Vol.49 No.7: 910-916

DOI: 10.3969/j.issn.1003-5060.2026.07.007

Authors

ZHANG Ru $ ^{1,2} $, YAO Bangben $ ^{2} $, HONG Ting $ ^{1} $, CHEN Zhaoran $ ^{1} $, XU Jianguo $ ^{1} $, CHEN Wei $ ^{1} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Anhui Institute of Product Quality Supervision and Inspection, Hefei 230051, China)

Abstract and Keywords

Abstract: This paper selects specific primers and probes from six common raw materials for making vermicelli, including sweet potato, cassava, mung bean, pea, potato, and corn, to establish a quantitative real-time polymerase chain reaction (RT-qPCR) method for identifying adulterated ingredients in vermicelli. The method of TaqMan probe is to connect the fluorescence group FAM at 5' and the quenching group TAMAR at 3'. When the probe is hydrolyzed, the fluorescence group separates from the quenching group, and the fluorescence generated by the fluorescence group can be detected by the instrument. The results showed that the relative sensitivity of the method was 1%, and the absolute sensitivity was $ 10^{-2} $ ng/ $ \mu $L. It can be applied to the identification of sweet potato, cassava, mung bean, pea, potato and corn-derived components in 60 commercially available vermicelli. The TaqMan probe-based RT-qPCR detection takes a short reaction time, and has the advantages of high sensitivity, excellent specificity, and accurate quantification, making it an effective tool for detecting adulterants in vermicelli.

Keywords: vermicelli adulteration; quantitative real-time polymerase chain reaction(RT-qPCR); probe method

作者信息

张茹 $ ^{1,2} $，姚帮本 $ ^{2} $，洪婷 $ ^{1} $，陈赵然 $ ^{1} $，徐建国 $ ^{1} $，陈伟 $ ^{1} $

(1. 合肥工业大学食品与生物工程学院, 安徽合肥 230601; 2. 安徽省产品质量监督检验研究院, 安徽合肥 230051)

摘要和关键词

摘要: 文章选取红薯、木薯、绿豆、豌豆、马铃薯、玉米6种常见制作粉丝原料的特异性引物和探针，建立实时荧光定量聚合酶链式反应（polymerase chain reaction，PCR）方法用于粉丝中掺假成分的鉴别。水解探针（TaqMan探针）的设计方法是在5'端连接荧光基团羧基荧光素（FAM），3'端连接猝灭基团羧基四甲基罗丹明（TAMAR），当探针发生水解时，荧光基团与淬灭基团分离，荧光基团所产生的荧光即可被仪器检测到。结果表明，该方法的相对灵敏度为1%，绝对灵敏度为 $ 10^{-2} $ ng/ $ \mu $L，可应用于市售60种粉丝中的红薯、木薯、绿豆、豌豆、马铃薯、玉米源性成分的鉴别。基于TaqMan探针实时荧光定量PCR检测方法反应耗时短，具有灵敏度高、特异性好、定量准确等优点，可以作为粉丝中源性成分掺假检测的有效方法之一。

关键词: 粉丝掺假；实时荧光定量聚合酶链式反应；探针法

Funding

安徽省科技攻关计划资助项目（2022f04020003）；安徽省自然科学基金资助项目（202104a07020008）

合肥工业大学学报(自然科学版)

Navigation