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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Independent C line modified test strips for rapid and stable salbutamol detection

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独立 C 线改进的试纸条快速稳定检测沙丁胺醇

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Nov.2025, Vol.48 No.11: 1501-1507

DOI: 10.3969/j.issn.1003-5060.2025.11.010

Authors

PENG Niuniu, CHEN Wei, CHEN Qi, WANG Hang, QU Wei, YANG Qingli

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Salbutamol, a form of lean meat powder, can eventually harm people through the food chain. Therefore, a convenient and rapid method is needed for field detection. This paper presents an independent C line method based on traditional colloidal gold immunolateral chromatography, which introduces independent C line antigen and independent C line antibody. The competition between C and T lines in the traditional test strips is avoided. Compared with the traditional test strip system, the stability of the optimized test strip is higher. The detection limit of salbutamol on the test strip is $ 5.0 \mu g/L $, and the method only takes 5 min, which has the potential for reliable and rapid field detection of salbutamol in food safety monitoring applications.

Keywords: salbutamol; independent C line; point-of-care testing; stability

作者信息

彭妞妞，陈伟，陈琦，王杭，屈玮，杨晴丽

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 沙丁胺醇作为瘦肉精的一种, 最终会通过食物链对人体造成危害, 因此, 需要一种便捷、快速的方法用于现场检测。文章在传统胶体金免疫侧向层析的基础上提出一种独立 C 线的检测方法, 该方法引入独立 C 线抗原和独立 C 线抗体, 避免了传统试纸条中 C 线、T 线之间存在竞争的情况。对该试纸条进行优化后, 与传统试纸条体系相比, 稳定性更高。检测结果表明, 该试纸条对沙丁胺醇的检测限为 $ 5.0 \mu g/L $, 且仅需 5 min 即可完成检测, 在食品安全监测应用中具有可靠、快速现场检测沙丁胺醇的潜力。

关键词: 沙丁胺醇；独立C线；现场快速检测；稳定性

Funding

国家自然科学基金资助项目（32172295）

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