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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Construction of overexpression vector of PuFAD8 gene and its genetic transformation in tomato(Solanum lycopersicum)

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PuFAD8 基因过表达载体的构建及其在番茄中的遗传转化

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Mar.2023, Vol.46 No.3: 392-396

DOI: 10.3969/j.issn.1003-5060.2023.03.017

Authors

SONG Huihui, YAN Longfei, GOU Shasha, YAO Gaifang, HU Kangdi, ZHANG Hua

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Fatty acid desaturase 8(FAD8) is a member of the FAD gene family, which can catalyze the synthesis of unsaturated fatty acids and affect fruit aroma. In order to further study the function of PuFAD8 gene and its relationship with volatile compounds in tomato (Solanum lycopersicum) fruit, the overexpression vector of tomato PuFAD8 gene was constructed. The overexpression plant of tomato PuFAD8 gene was obtained by Agrobacterium-mediated transformation and plant tissue culture. The results showed that the overexpression vector of tomato PuFAD8 gene was successfully constructed and tomato overexpression plants were successfully obtained in order to further study the molecular function of PuFAD8 gene and clarify the regulation mechanism of volatile compounds during tomato fruit ripening.

Keywords: tomato(Solanum lycopersicum); PuFAD8 gene; overexpression; vector construction; aroma

作者信息

宋慧慧，颜龙飞，苟莎莎，姚改芳，胡康棣，张华

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 脂肪酸去饱和酶8(fatty acid desaturase 8, FAD8)是FAD基因家族成员, 可参与催化不饱和脂肪酸的合成进而影响果实香气。为进一步研究PuFAD8基因的功能及其与番茄果实挥发性物质的关系, 文章构建了模式植物番茄PuFAD8基因的过表达载体, 通过农杆菌介导遗传转化、植物组织培养技术获得番茄PuFAD8基因过表达植株。结果表明, 番茄PuFAD8基因过表达载体构建成功, 并获得番茄过表达植株, 以便进一步研究该基因的分子功能, 为明确其在番茄果实成熟过程中挥发性物质的调控机制奠定了基础。

关键词: 番茄；PuFAD8基因；过表达；载体构建；香气

Funding

国家自然科学基金资助项目（31970312；32170315）

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