Methylene blue mediated polymerase chain reaction on electrochemical sensing interface for detection of Salmonella typhimurium

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Dec.2023, Vol.46 No.12: 1706-1710

DOI: 10.3969/j.issn.1003-5060.2023.12.018

Authors

GAO Jie $ ^{1} $, CHEN Wei $ ^{1} $, HONG Ting $ ^{2} $, YAO Bangben $ ^{2} $, YAO Li $ ^{1} $, XU Jianguo $ ^{1} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Anhui Institute of Product Quality Supervision and Inspection, Hefei 230051, China)

Abstract and Keywords

Abstract: This paper proposed a method for rapid detection of Salmonella typhimurium in milk. The target amplified products of Salmonella typhimurium can be measured by polymerase chain reaction (PCR) amplification on the electrochemical sensing interface with the assistance of intercalating redox reporter methylene blue. The proposed screen-printed electrode based electrochemical detection can be completed within 120 min with a linear range of $ 3 \times 10^{1} - 3 \times 10^{7} $ CFU/mL and it is not affected by the milk matrix. This method is fast and simple and specific, which is expected to be used in the on-site rapid detection of foodborne pathogens.

Keywords: Salmonella typhimurium; methylene blue; polymerase chain reaction(PCR) amplification; electrochemistry; rapid detection

作者信息

高洁 $ ^{1} $，陈伟 $ ^{1} $，洪婷 $ ^{2} $，姚帮本 $ ^{2} $，姚丽 $ ^{1} $，徐建国 $ ^{1} $

(1. 合肥工业大学食品与生物工程学院, 安徽合肥 230601; 2. 安徽省产品质量监督检验研究院, 安徽合肥 230051)

摘要和关键词

摘要: 文章研究一种可用于快速检测牛奶中鼠伤寒沙门氏菌（Salmonella typhimurium）的方法，通过在聚合酶链式反应（polymerase chain reaction，PCR）扩增体系中加入嵌入式氧化/还原指示剂亚甲基蓝可以实现对扩增产物的电化学检测。文中所提出的基于丝网印刷电极的电化学检测方法可在120 min内完成对鼠伤寒沙门氏菌的检测，线性范围为 $ 3 \times 10^{1} \sim 3 \times 10^{7} $ CFU/mL，且不受牛奶基质影响。该方法快速、简便、特异性好，有望应用于食源性致病菌现场快速检测。

关键词: 鼠伤寒沙门氏菌；亚甲基蓝；聚合酶链式反应（PCR）扩增；电化学；快速检测

Funding

国家自然科学基金资助项目(32172295); 安徽省自然科学基金资助项目(2208085QB56) 和广东省食品质量与安全重点实验室开放基金资助项目(2020KF001)

合肥工业大学学报(自然科学版)

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