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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Construction of VIGS silencing vector of SlERF-H6 gene in tomato

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番茄 SlERF-H6 基因 VIGS 沉默载体构建

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Sept.2024, Vol.47 No.9: 1232-1235, 1242

DOI: 10.3969/j.issn.1003-5060.2024.09.011

Authors

LI Lixia, HU Kangdi, YAO Gaifang, ZHANG Hua

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Tomato (Solanum lycopersicum) has high nutritional value and is deeply loved by consumers. At present, tomato has become one of the widely planted cash crops. Ethylene response factor (ERF) is a kind of plant-specific transcription factor. ERF plays an important role in a variety of physiological processes, such as plant morphogenesis, stress response, maturation and senescence, hormone signal transduction and metabolite regulation. In this paper, taking the SlERF-H6(LOC101259323) gene of tomato as the research object, the specific region silencing sequence of SlERF-H6 gene was obtained by analyzing the sequence of SlERF-H6 gene. The virus-induced gene silencing (VIGS) vector of tomato SlERF-H6 was successfully constructed by homologous recombination technique. The purpose of this study is to provide a reference for further exploring the role of tomato SlERF-H6 gene in fruit ripening.

Keywords: tomato(Solanum lycopersicum); virus-induced gene silencing(VIGS); SlERF-H6 gene; homologous recombination; vector construction

作者信息

李立霞，胡康棣，姚改芳，张华

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 番茄(Solanum lycopersicum)具有较高的营养价值，深受消费者的喜爱，目前番茄已经成为广泛种植的经济作物之一。乙烯响应因子(ethylene response factor, ERF)是一类植物特有的转录因子。ERF在植物形态发生、逆境响应、成熟衰老、激素信号转导和代谢物调节等多种生理过程中起重要的调节作用。文章以番茄的SlERF-H6(LOC101259323)基因为研究对象，通过对SlERF-H6的基因序列进行分析，获得该基因的特异性区域沉默序列。利用同源重组技术成功构建了番茄SlERF-H6病毒诱导的基因沉默(virus-induced gene silencing, VIGS)载体。为进一步探究番茄SlERF-H6基因在果实成熟中的作用提供参考。

关键词: 番茄；病毒诱导的基因沉默（VIGS）；SlERF-H6基因；同源重组；载体构建

Funding

国家自然科学基金资助项目（31970200；31970312；32170315）

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