Preparation and properties of Lactobacillus reuteri enteric-coated granules loaded on wheat straw

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Nov.2024, Vol.47 No.11: 1533-1539

DOI: 10.3969/j.issn.1003-5060.2024.11.015

Authors

CHAI Jiatai $ ^{1} $, LI Xiaowen $ ^{1,2} $, ZHAN Jinsuo $ ^{1} $, ZHANG Chuanwei $ ^{1} $, YAO Risheng $ ^{1} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Anhui Anson Biochemical Technology Co., Ltd., Hefei 231299, China)

Abstract and Keywords

Abstract: In order to develop new embedding carrier materials for probiotic preparations and solve the problem of poor thermal stability of existing products, wheat straw was used as the carrier material to load Lactobacillus reuteri, and then the wheat straw loaded with Lactobacillus reuteri was granulated and coated to prepare enteric granules, and their related properties were studied. The results showed that when the Lactobacillus reuteri granules loaded on wheat straw were heat-treated at 50 ℃ and 121 ℃ for 60 min, the viable bacterial counts of Lactobacillus reuteri were respectively maintained at 2.8×10^{8} CFU/g and 6.0×10^{7} CFU/g. In the control group, when the Lactobacillus reuteri granules loaded on porous starch were heat-treated at 50 ℃ for 60 min, the amount of viable Lactobacillus reuteri was 2.3×10^{7} CFU/g. When the Lactobacillus reuteri granules loaded on porous starch were heat-treated at 121 ℃ for 60 min, almost all Lactobacillus reuteri were inactivated. At the same time, it was also found that the viable amount of Lactobacillus reuteri loaded on wheat straw and prepared into enteric-coated granules was 2.4×10^{8} CFU/g, and it had a certain slow-release effect in artificial intestinal fluid. In the control group, the viable amount of Lactobacillus reuteri loaded on porous starch and prepared into enteric-coated granules was 2.6×10^{7} CFU/g, which showed no slow-release effect.

Keywords: wheat straw; loading; Lactobacillus reuteri; enteric-coated granules; heat resistance

作者信息

柴佳太 $ ^{1} $，李效文 $ ^{1,2} $，詹进锁 $ ^{1} $，张传伟 $ ^{1} $，姚日生 $ ^{1} $

(1. 合肥工业大学食品与生物工程学院，安徽合肥 230601；2. 安徽安生生物化工科技有限责任公司，安徽合肥 231299)

摘要和关键词

摘要: 为开发益生菌制剂用的新包埋载体材料,以解决现有技术产品热稳定性差的难题,文章采用麦秸秆做载体材料负载罗伊氏乳杆菌,对负载罗伊氏乳杆菌的麦秸秆进行制粒包衣成肠溶颗粒剂,并对其相关性能进行研究。结果表明:麦秸秆负载罗伊氏乳杆菌颗粒分别在50、121℃下热处理60min,罗伊氏乳杆菌活菌量分别保持在 $ 2.8\times10^{8} $、 $ 6.0\times10^{7} $ CFU/g;而对照组多孔淀粉负载罗伊氏乳杆菌颗粒在50℃下热处理60min,罗伊氏乳杆菌活菌量为 $ 2.3\times10^{7} $ CFU/g,在121℃下热处理60min,罗伊氏乳杆菌几乎全部失活;用麦秸秆负载罗伊氏乳杆菌制备成肠溶颗粒剂后的活菌量为 $ 2.4\times10^{8} $ CFU/g,且在人工肠液中具有一定的缓释效果;而用对照组多孔淀粉负载罗伊氏乳杆菌制成肠溶颗粒剂后的活菌量为 $ 2.6\times10^{7} $ CFU/g,在人工肠液中不具有缓释效果。因此麦秸秆负载罗伊氏乳杆菌颗粒具有较好的耐热性能和一定的缓释效果。

关键词: 麦秸秆；负载；罗伊氏乳杆菌；肠溶颗粒剂；耐热

Funding

安徽省科技重大专项资助项目（202003a05020060）

合肥工业大学学报(自然科学版)

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