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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Role of transcription factor SlSPL3 in pathogen stress

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转录因子 SlSPL3 在病原菌胁迫中的作用

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Jun.2025, Vol.48 No.6: 791-797

DOI: 10.3969/j.issn.1003-5060.2025.06.012

Authors

LU Ruoxi, MIAO Min, FAN Yezhen, SONG Wu, ZHU Hongyan, WU Zirui

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: SPL family genes play important roles in plant growth and development and abiotic responses, but their roles in plant resistance to pathogenic microbial attack are unknown. In this study, a pBI121-35S::SlSPL3 gene overexpression vector was constructed and SlSPL3 overexpression transgenic plants were obtained using a tomato genetic transformation method. It was found that overexpression of the SlSPL3 gene significantly enhanced the resistance to Pst DC3000 in tomato. The SlSPL3 interacted with ubiquitin molecules and was subject to ubiquitinated degradation, suggesting that it may act in concert with the resistance protein SlNBRP1. This study provides a theoretical basis and genetic resources for the genetic improvement of crop disease resistance.

Keywords: tomato; SlSPL3 gene; Pst DC3000; ubiquitination

作者信息

卢若兮，苗敏，范叶珍，宋武，祝红艳，吴子睿

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: SPL 家族成员在植物生长发育和非生物响应中发挥重要的作用,但其在植物抗病原微生物侵害方面的作用尚不清楚。文章构建了 pBI121-35S::StSPL3 基因过表达载体,并利用番茄遗传转化方法获得 Sl-SPL3 过表达转基因植株,发现过量表达 SlSPL3 基因能够显著增强番茄对丁香假单胞菌蕃茄致病变种 Pst DC3000 的抗性,SlSPL3 与泛素分子互作并受到泛素化降解,表明其可能与抗病蛋白 SlNBRP1 协同发挥作用。该研究为作物抗病遗传改良提供了理论基础和基因资源。

关键词: 番茄；SlSPL3基因；Pst DC3000；泛素化

Funding

国家自然科学基金资助项目（31970345）

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