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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Role of motB and fliC genes in the motility of Escherichia coli

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motB 和 fliC 基因在大肠杆菌运动中的作用

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Jul.2025, Vol.48 No.7: 938-944, 950

DOI: 10.3969/j.issn.1003-5060.2025.07.012

Authors

HUANG Chengyun, LIU Jian, WANG Fangbin

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Peritrichous bacteria can provide motivation for own motility by employing flagellar motor to turn the flagella. In this paper, Escherichia coli was used as model organism, and the Escherichia coli genome was modified by CRISPR-Cas9 gene editing technology to construct the Escherichia coli strains without motB and fliC, respectively. The rotation of single flagellar motor was studied by bright field microscopy; the individual bacterial swimming motility was studied by dark field microscopy; the swimming motility of bacterial population was studied by using soft agar plates. The results showed that the strains of Escherichia coli without motB and fliC lost the ability of motor rotation and bacterial motility. The motility of Escherichia coli was restored when plasmids pJY7 expressing motB and pKAF131 expressing fliC were transformed to the cells. The results suggested the role of motB and fliC in the motility of Escherichia coli.

Keywords: Escherichia coli; flagella; flagellar motor; motB gene; fliC gene; CRISPR-Cas9 gene editing technology

作者信息

黄承允，刘健，王芳彬

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 周身含有鞭毛的细菌可通过鞭毛马达带动鞭毛转动,为自身运动提供动力。文章以大肠杆菌为模式生物,通过CRISPR-Cas9基因编辑技术对大肠杆菌基因组进行改造,分别构建motB和fliC基因缺失的大肠杆菌菌株;利用明场显微技术研究单个鞭毛马达的转动,利用暗场显微技术研究单个细菌的游动,利用软琼脂平板研究细菌的群体游动。结果表明:motB和fliC基因缺失的大肠杆菌失去了马达转动和细胞运动的能力;通过质粒pJY7和pKAF131分别回补motB和fliC基因,恢复了大肠杆菌的运动能力,进一步明确了motB和fliC基因在大肠杆菌运动中的作用。

关键词: 大肠杆菌；鞭毛；鞭毛马达；motB基因；fliC基因；CRISPR-Cas9基因编辑技术

Funding

国家自然科学基金资助项目（11804072）

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