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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Functional study of tomato APR1 gene in sulfur metabolic pathway

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番茄 APR1 基因在硫代谢通路中的功能研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Aug.2025, Vol.48 No.8: 1085-1091

DOI: 10.3969/j.issn.1003-5060.2025.08.012

Authors

WANG Siyue, ZHANG Hua, GUO Wentong, YU Yue, HU Kangdi, YAO Gaifang

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: In this paper, bioinformatics analysis of APR gene family members in different plant species was conducted. Using tomato as experimental material, SlAPR1 gene editing vector was successfully constructed by CRISPR-Cas9 gene editing technology and transferred into Agrobacterium tumefaciens receptor cells EHA105. SlAPR1 gene editing plants were successfully constructed using histopathogenesis technology, and it was found that the endogenous hydrogen sulfide content in their roots was significantly lower than that of wild-type tomato plants, indicating that the reduced expression of SlAPR1 gene inhibits the production of endogenous hydrogen sulfide, which is an important basis for studying the function and mechanism of SlAPR1 gene in tomato sulfur metabolic pathway.

Keywords: tomato; SlAPR1 gene; bioinformatics; gene editing technology; hydrogen sulfide

作者信息

王思月，张华，郭文通，余越，胡康棣，姚改芳

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 文章对不同植物物种中 APR 基因家族成员进行生物信息学分析，以番茄为实验材料，利用 CRISPR-Cas9 基因编辑技术成功构建 SlAPR1 基因编辑载体，并转入农杆菌感受态细胞 EHA105。采用组培技术成功构建 SlAPR1 基因编辑植株，检测发现该植株根部含内源硫化氢量明显低于野生型番茄植株，说明 SlAPR1 基因表达量降低会抑制内源硫化氢的产生。该结果为研究 SlAPR1 基因在番茄硫代谢通路中的功能及作用机制奠定了基础。

关键词: 番茄；SlAPR1基因；生物信息学；基因编辑技术；硫化氢

Funding

国家自然科学基金资助项目（32170315）

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