Abstract: In this paper, bone marrow derived mast cell (BMMC) was cultured for five weeks with 10 ng/mL, 50 ng/mL and 200 ng/mL stem cell factor (SCF) and the mRNA levels of mast cell protease 4 (MCP4), MCP5, MCP6 and MCP7 were detected every week during the culture process. The results showed that the mRNA expression level of MCP was affected by culture duration and SCF concentration. The mRNA expression level of $ Mc_{pt4} $ significantly increased with the increase of SCF concentration, and the high expression was induced in the third week under high-concentration SCF culture conditions. The mRNA expression level of $ Mc_{pt5} $ was high under medium-concentration SCF culture conditions and reached a maximum in the fourth week. The mRNA expression level of $ Mc_{pt6} $ was high under low-concentration SCF culture conditions and reached a maximum in the third week. The mRNA expression level of $ Mc_{pt7} $ was similar to that of $ Mc_{pt4} $, which significantly increased with the increase of SCF concentration, and was the highest in the fourth week under high-concentration SCF culture conditions. The results provide a new basis for studying the activity and physiological functions of BMMC.
Keywords: bone marrow derived mast cell (BMMC); stem cell factor (SCF); mast cell protease (MCP); mast cell tryptase (MCT)
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