Abstract: Marigold(Tagetes erecta) is a major plant resource for natural pigments extraction and also a widely used plant for horticulture cultivation. The hybrid $ F_{1} $ displays advantaged curds but it is difficult to set seeds for offspring. In this study, three cultivars of Tagetes erecta, Juwang, Taishan and Discovery, were used for $ F_{1} $ plantlet tissue culture. Their stem segments with axillary buds were selected as explants to detect the effects of media with different phytohormone combinations on these varieties of Tagetes erecta. The results showed that Juwang was easier to induce regenerated shoots, and the optimal induction medium was MS, 1.00 mg/L 6-BA, 0.05 mg/L NAA, 30 g/L sucrose and 8 g/L agar to indicate 94.44% of regeneration rate and 5.60% of vitrification rate after adding filter paper. The addition of 0.2 g/L timentin could inhibit the growth of endophyte in stem segments. The optimal rooting medium was 1/2MS, 0.1 mg/L IAA, 30 g/L sucrose and 8 g/L agar to indicate 100% of rooting rate and well-developed roots in regenerated plantlets. In the present study, an efficient tissue culture regeneration system was established in three marigold cultivars, which will provide a research basis for rapid propagation and genetic improvement in expensive high-quality marigold materials.
Keywords: marigold (Tagetes erecta); tissue culture; stem segment with axillary bud; regenerated plantlet; rapid propagation
合肥工业大学学报自科版