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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Response of antibiotic-resistant bacteria in water supply networks to sodium hypochlorite and water flow patterns

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供水管网中抗生素抗性细菌对次氯酸钠和水流流态的响应

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Nov.2025, Vol.48 No.11: 1572-1578

DOI: 10.3969/j.issn.1003-5060.2025.11.020

Authors

MA Jinshan, LIU Li, SHAN Rongrong, WEI Changlin, CHEN Guowei

(School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei 230009, China)

Abstract and Keywords

Abstract: The misuse of antibiotics in recent years has led to the widespread dissemination of antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs) in the water environment, which seriously threatens the safety of drinking water quality. This paper investigated the effects of sodium hypochlorite and water flow patterns on the distribution characteristics of ARBs by constructing a simulated experimental setup for water supply network, and established their correlation with bacterial aggregation behavior. The results showed that the dosing of sodium hypochlorite ( $ \rho \leq 1.0 \, mg/L $) decreased the abundance of ARBs in biofilm and water body, but 1.0 mg/L sodium hypochlorite increased the percentage of ARBs, and this trend was more significant under transitional flow condition. Spearman correlation analysis revealed a significant positive correlation between the ARBs in biofilm and bulk water and the heterotrophic plate count (HPC), which indicated that increasing biomass, especially its aggregation behavior, contributed to the proliferation and spreading of ARBs under different disinfectants and water flow patterns. In addition, the results of population analysis showed that the flow pattern and sodium hypochlorite had a certain effect on the population structure of attached and suspended bacteria, especially on the relative abundance of Ascomycetes, which in turn regulated the spatial distribution of ARBs.

Keywords: water flow pattern; sodium hypochlorite disinfection; antibiotic-resistant bacteria(ARBs); biofilm; water supply network

作者信息

马金山，刘丽，单蓉蓉，韦长林，陈国炜

（合肥工业大学土木与水利工程学院，安徽合肥230009）

摘要和关键词

摘要: 近年来, 抗生素的滥用导致水环境中抗生素抗性细菌(antibiotic-resistant bacteria, ARBs)和抗生素抗性基因(antibiotic resistance genes, ARGs)的广泛扩散, 严重威胁饮用水水质安全。文章通过搭建给水管网模拟实验装置, 研究次氯酸钠和水流流态对抗生素抗性细菌分布特征的影响, 构建其与细菌聚集行为的关联。结果表明, 次氯酸钠的投加( $ \rho\leq1.0\ mg/L $)降低了生物膜和水体中ARBs的丰度, 但1.0 mg/L次氯酸钠会提高ARBs的占比, 特别当水流流态为过渡流时, 该趋势更为显著。Spearman相关性分析表明, 生物膜和主体水中ARBs与异养菌数(heterotrophic plate count, HPC)呈显著正相关, 说明不同消毒剂与水流流态下增大生物量特别是其聚集行为有助于ARBs的增殖和扩散。此外, 种群分析结果表明流态及次氯酸钠对附着与悬浮细菌种群结构有一定的影响, 尤其对变形菌纲的相对丰度影响较大, 进而调控ARBs的空间分布。

关键词: 水流流态；次氯酸钠消毒；抗生素抗性细菌（ARBs）；生物膜；供水管网

Funding

安徽省自然科学基金资助项目（2208085US13）

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