Research on the augmentation of sequencing batch reactor with N-acyl-homoserine lactones to counteract nitrification process under high salinity

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Aug. 2026, Vol. 49 No. 8: 1015-1020, 1088

DOI: 10.3969/j.issn.1003-5060.2026.08.002

Authors

WANG Yinghao $ ^{1} $, WANG Jin $ ^{1,2} $, MA Ding $ ^{1,2} $, JIANG Yifan $ ^{1} $, ZHAI Hui $ ^{1} $, YUE Zhengbo $ ^{1,2} $

(1. School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China; 2. Anhui Engineering Research Center of Industrial Wastewater Treatment and Resource Recovery, Hefei 230009, China)

Abstract and Keywords

Abstract: In this paper, exogenous N-acyl-homoserine lactones(AHLs) were added to a sequencing batch reactor(SBR) to promote quorum sensing(QS). The effects of AHLs with different carbon chain lengths(C4-L-HSL, C6-L-HSL, and C8-L-HSL) and different substituent structures(C6-L-HSL, C6-DL-HSL, and 3-oxo-C6-HSL) on the SBR system's ability to cope with high salinity stress and the changes in sludge properties were systematically investigated. Experiments showed that under 5% and 8% salinity stress for the first ten days, compared to 2% salinity, the average $ NH_{4}^{+}-N $ removal rate decreased by 6.06% and 18.32%, respectively, with significant $ NO_{2}^{-}-N $ accumulation in the effluent. Among all groups, the group with added 3-oxo-C6-HSL best alleviated salinity stress, reducing the $ NO_{2}^{-}-N $ accumulation peak by 39.54% and 58.09% under 5% and 8% salinity, respectively, and showed remarkable improvement in $ NH_{4}^{+}-N $ removal and $ NO_{2}^{-}-N $ accumulation. Moreover, the analysis of extracellular polymeric substances(EPS) showed that the protein and polysaccharide mass concentrations in the experimental group were increased by 14.4%-38.4% and 26.0%-41.0%, respectively. The $ UV_{254} $ data of EPS showed that the content of large molecular organic matter and aromatic compounds in the experimental group was higher, indicating that the metabolic pathways in the experimental group were more diverse and the products were more complex. Analysis of the microbial community indicated that Proteobacteria was the dominant phylum in the system. community indicated that Proteobacteria was the dominant phylum in the system.

Keywords: quorum sensing(QS); N-acyl-homoserine lactones(AHLs); nitrification; salinity stress

作者信息

王莹皞 $ ^{1} $，王进 $ ^{1,2} $，马丁 $ ^{1,2} $，江一凡 $ ^{1} $，翟辉 $ ^{1} $，岳正波 $ ^{1,2} $

(1. 合肥工业大学资源与环境工程学院，安徽合肥 230009；2. 安徽省工业废水处理与资源化工程研究中心，安徽合肥 230009)

摘要和关键词

摘要: 文章通过在序批式反应器（sequencing batch reactor，SBR）中添加外源性N-酰基-高丝氨酸内酯（AHLs）以促进体系内群体感应现象的发生，系统探究了不同碳链长度（C4-L-HSL、C6-L-HSL、C8-L-HSL）和不同取代基结构（C6-L-HSL、C6-DL-HSL、3-oxo-C6-HSL）的AHLs对SBR系统应对高盐度胁迫能力的影响及污泥性质变化。结果表明：相较于2%盐度，5%、8%盐度胁迫的前10 d， $ NH_{4}^{+}-N $的平均去除率分别下降了6.06%、18.32%，且出水中有大量 $ NO_{2}^{-}-N $的积累；在所有组别中，添加3-oxo-C6-HSL的实验组在应对盐度胁迫上表现最佳，周期内 $ NO_{2}^{-}-N $积累峰值在5%盐度、8%盐度分别低于对照组39.54%、58.09%， $ NH_{4}^{+}-N $去除率提升和 $ NO_{2}^{-}-N $积累改善最为明显。此外，对污泥胞外聚合物（extracellular polymeric substances，EPS）的提取分析发现，实验组的蛋白和多糖质量浓度分别提高了14.4%~38.4%和26.0%~41.0%。EPS的 $ UV_{254} $数据表明，实验组中大分子有机物及芳香族化合物质量浓度较高，说明其代谢途径更为丰富，产物也更为复杂。微生物群落分析表明变形杆菌门（Proteobacteria）是该系统中主要的优势菌门。

关键词: 群体感应；N-酰基-高丝氨酸内酯（AHLs）；硝化；盐度胁迫

Funding

安徽省自然科学基金青年科学基金资助项目（2308085QE187）

合肥工业大学学报(自然科学版)

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