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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Rapid detection of Ara h1 based on circular aptamers

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基于环状核酸适体的 Ara h1 快速检测

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Jul. 2026, Vol.49 No.7: 924-928, 975

DOI: 10.3969/j.issn.1003-5060.2026.07.009

Authors

FENG Junmei, MAO Yu, FANG Peng, SUN Long, YAO Lili

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: In this paper, a fluorescent biosensor based on circular aptamer and magnetic graphene oxide (MGO) platform was constructed for the rapid and accurate label-free detection of peanut allergen protein Ara h1 in the samples. Circular aptamer specifically targeting Ara h1 was adsorbed onto MGO by $ \pi-\pi $ stacking. When Ara h1 presented in the samples, the circular aptamer bound to Ara h1 to form a complex, which in turn desorbed from the MGO surface. MGO was then removed by magnetic separation. The Ara h1-circular aptamer complex was denatured by heating to detach the circular aptamer, which could then be further used as a template for rolling circle amplification (RCA). Finally, the fluorescent signals could be generated by adding Sybr Gold nucleic acid dye. The fluorescent biosensor has excellent specificity and sensitivity, with a detection limit as low as 0.02 $ \mu $mol/L and a linear range of 0.02–1.70 $ \mu $mol/L. The biosensor can be used for the rapid detection of Ara h1 in real samples, which is simple to operate and does not require chemical modification, holding great potential for food allergen detection.

Keywords: peanut allergen protein Ara h1; circular aptamer; rolling circle amplification(RCA); magnetic graphene oxide(MGO); rapid detection

作者信息

凤君妹，毛瑜，方鹏，孙龙，姚丽丽

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 为了能精准、快速检测出样品中的花生过敏源 Ara h1, 文章设计了一种基于环状核酸适体和磁性氧化石墨烯(magnetic graphene oxide, MGO)平台的无标记快速检测花生过敏原 Ara h1 的荧光传感器。特异性靶向 Ara h1 的环状核酸适体通过 $ \pi-\pi $ 堆积吸附在 MGO 上, 当样品中含有 Ara h1 时, 环状核酸适体与 Ara h1 结合形成复合物, 进而从 MGO 表面解吸附; 磁性分离去除 MGO, 再通过加热使 Ara h1 变性脱离其环状核酸适体, 此时环状核酸适体可以进一步作为滚环扩增(rolling circle amplification, RCA)的模板进行 RCA 反应, 加入 Sybr Gold 核酸染料即可产生荧光信号。该荧光传感器具有很好的特异性和灵敏度, 检测限低至 $ 0.02 \mu mol/L $, 检测线性范围为 $ 0.02 \sim 1.70 \mu mol/L $。该荧光传感器可用于实际样品中 Ara h1 的快速检测, 且操作简单、无需化学修饰, 在食物过敏原检测中有良好的应用前景。

关键词: 花生过敏原 Ara h1；环状核酸适体；滚环扩增（RCA）；磁性氧化石墨烯（MGO）；快速检测

Funding

国家重点研发计划资助项目（2020YFC1606802）；安徽省自然科学基金青年基金资助项目（2108085QC139）

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