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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Effect of activated sludge on microalgae aggregation and bacterial community response

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活性污泥对微藻聚集影响及细菌群落响应研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Dec.2025, Vol.48 No.12: 1629-1637

DOI: 10.3969/j.issn.1003-5060.2025.12.008

Authors

DING Yulong, CHEN Guowei, SHI Huasheng, WU Xuefei, SU Kuizu, LIU Li

(School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei 230009, China)

Abstract and Keywords

Abstract: To address the issue of microalgae harvesting, this study took Chlamydomonas microsphaera as the research subject to investigate the effect of activated sludge on microalgae aggregation and the impact of microalgae aggregation on the bacterial community structure of the sludge, thereby revealing the mechanisms underlying the formation of bacterial-algal aggregates. The results showed that the presence of sludge bacteria significantly promoted the aggregation of Chlamydomonas microsphaera and its aggregation rate increased with the increase of the number of sludge bacteria. The aggregation rate of Chlamydomonas microsphaera in mixed culture was higher than that in pure culture (12.94%), and the aggregation rate could reach 62.92% at the ratio of algae to bacteria of 1:10. At the same time, the extracellular polymeric substances (EPS) secretion of the bacterial-algal aggregates increased about 163.38 mg/L compared to the pure culture of microalgae. Analysis of the population change of sludge bacteria showed that Pseudomonas and Rhizobium played an important role in promoting the aggregation of Chlamydomonas microsphaera.

Keywords: Chlamydomonas microsphaera; activated sludge; microalgae aggregation

作者信息

丁雨龙，陈国炜，施华升，吴雪菲，苏馈足，刘丽

（合肥工业大学土木与水利工程学院，安徽合肥230009）

摘要和关键词

摘要: 针对微藻收集问题，文章以小球衣藻（Chlamydomonas microsphaera）为研究对象，探究活性污泥细菌对微藻聚集的影响和微藻聚集对污泥细菌群落结构的影响，揭示菌藻聚集体形成机制。结果表明：污泥细菌的出现能显著促进小球衣藻的聚集，其聚集率随着污泥细菌数量的增加而上升，混合培养时小球衣藻聚集率比纯培养时（聚集率为12.94%）有较大提升，在藻菌细胞数量比为1：10时，聚集率可达62.92%；菌藻体系中菌藻聚集体的胞外聚合物（extracellular polymeric substances，EPS）分泌量比纯培养微藻上升约163.38 mg/L。对污泥细菌种群变化的分析显示，假单胞菌属Pseudomonas和根瘤菌属Rhizobium在促进小球衣藻聚集方面发挥着重要的作用。

关键词: 小球衣藻；活性污泥；微藻聚集

Funding

国家自然科学基金资助项目（42277298）

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