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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Protective effect and mechanism of bilobalide on depression induced by chronic stress in mice

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白果内酯对慢性应激导致小鼠抑郁的保护作用及机制研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Oct.2024, Vol.47 No.10: 1362-1367

DOI: 10.3969/j.issn.1003-5060.2024.10.010

Authors

YANG Chengying, HUI Ailing, WU Zeyu, ZHANG Wencheng

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: In order to clarify the protective effect of bilobalide(BB) on depressed mice as well as its mechanism, BB with a purity of 98.21% was prepared from 40% BB by molecular imprinting solid phase extraction (MISPE). Thirty-six ICR mice aged six-to-eight weeks were randomly divided into six groups: control group, model group, fluoxetine group(10 mg/kg), low, middle and high dose groups of BB(3.5, 7.0 and 14.0 mg/kg). Except for the control group, the depression models of mice were established by chronic unpredictable mild stress (CUMS) model. In the course of the experiment, the changes of depression in mice after BB intervention were observed, and the body weight of mice was recorded. At the end of the experiment, the contents of inflammatory factors in serum, monoamine neurotransmitters in brain and brain-derived neurotrophic factor(BDNF) in hippocampus were measured. The results showed that BB could improve the depression of mice, significantly reduce the contents of tumor necrosis factor- $ \alpha $(TNF- $ \alpha $) and interleukin-6(IL-6), increase the contents of

Keywords: bilobalide(BB); chronic unpredictable mild stress(CUMS) model; antidepressant; monoamine neurotransmitters; brain-derived neurotrophic factor(BDNF)

作者信息

杨程英，惠爱玲，吴泽宇，张文成

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 为了探究白果内酯（BB）对抑郁小鼠的保护作用及其作用机制，文章以40%白果内酯粗品为原料，通过分子印迹固相萃取法（molecular imprinting solid phase extraction，MISPE）制备纯度为98.21%白果内酯。采用6～8周龄ICR小鼠36只，随机分为对照组、模型组、氟西汀组（10 mg/kg）、低剂量组（BB 3.5 mg/kg）、中剂量组（BB 7.0 mg/kg）、高剂量组（BB 14.0 mg/kg）6组。除对照组外，其余组均通过慢性不可预测性轻度应激（chronic unpredictable mild stress，CUMS）模型构建小鼠抑郁模型。实验过程中观察白果内酯干预后小鼠抑郁行为的变化，并记录小鼠体质量。实验结束检测小鼠血清中炎症因子、脑组织内单胺类神经递质和海马区脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）的相对表达量。研究发现，白果内酯的干预使得小鼠抑郁情况得到改善，能够显著降低炎症因子肿瘤坏死因子- $ \alpha $（tumor necrosis factor- $ \alpha $，TNF- $ \alpha $）、白细胞介素-6（interleukin-6，IL-6）的质量浓度，提高单胺类神经递质多巴胺（DA）、去甲肾上腺素（NE）和5-羟色胺（5-HT）的质量比，并促进BDNF的表达。结果表明，白果内酯能够通过抑制炎症促进脑内单胺类神经递质的发生和BDNF的表达，从而发挥对抑郁小鼠的保护作用。

关键词: 白果内酯(BB); 慢性不可预测性轻度应激(CUMS)模型; 抗抑郁; 单胺类神经递质; 脑源性神经营养因子(BDNF)

Funding

安徽省自然科学基金资助项目（1808085MC77）；阜阳市科技重大专项揭榜挂帅类资助项目（FKJB202105）

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