Biodegradation of dimethyl phthalate by Phanerochaete chrysosporium and its mechanism

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Aug.2023, Vol.46 No.8: 1024-1031

DOI: 10.3969/j.issn.1003-5060.2023.08.003

Authors

GAO Siyan $ ^{1} $, CUI Kangping $ ^{1} $, LIU Rui $ ^{1} $, XU Xiangyang $ ^{1} $, CHEN Changbin $ ^{2} $, WANG Sanliu $ ^{2} $

(1. School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China; 2. Anhui Shuguang Chemical Group Co., Ltd., Anqing 246003, China)

Abstract and Keywords

Abstract: The biodegradation efficiency and mechanism of dimethyl phthalate(DMP) by Phanerochaete chrysosporium(P. chrysosporium), a kind of white-rot fungus, were investigated. Experiments showed that under optimal conditions (30 °C, pH of 5.6, initial DMP concentration of 10 mg/L), 75% of DMP was removed after seven days. When 5 g/L glucose was added into the system, the degradation efficiency of DMP was significantly increased to 95%. When 20 $ \mu $g/L $ H_{2}O_{2} $ or 27.0 mg/L $ Mn^{2+} $ were added into the system, the degradation efficiency of DMP was significantly increased to 100%. Moreover, peroxidase played an important role in the degradation process of DMP, and the degradation of DMP was more driven by manganese peroxidase (MnP). Fourier transform infrared spectrometer(FTIR) confirmed that amino, carboxyl, hydroxyl, and other reducing functional groups were involved in DMP removal. Based on liquid chromatography-mass spectrometry(LC-MS) analysis, several different DMP degradation intermediates were identified, and three possible degradation pathways of DMP were deduced.

Keywords: biodegradation; plasticizer; white-rot fungus; dimethyl phthalate(DMP); peroxidase

作者信息

高斯研 $ ^{1} $，崔康平 $ ^{1} $，刘睿 $ ^{1} $，许向阳 $ ^{1} $，陈长斌 $ ^{2} $，汪三六 $ ^{2} $

(1. 合肥工业大学资源与环境工程学院，安徽合肥 230009；2. 安徽曙光化工集团有限公司，安徽安庆 246003)

摘要和关键词

摘要: 文章以黄孢原毛平革菌(Phanerochaete chrysosporium)作为白腐真菌的代表, 研究其对邻苯二甲酸二甲酯(dimethyl phthalate, DMP)的降解效果与机理。实验结果表明, 在最佳条件(pH=5.6, 温度为30℃, DMP初始质量浓度为10 mg/L)下, 7 d后 DMP的去除率为75%。进一步优化反应条件发现: 体系中初始葡萄糖质量浓度为5 g/L时, 反应结束时DMP降解效率为95%; 当反应中加入20 $ \mu $g/L $ H_{2}O_{2} $ 或27.0 mg/L $ Mn^{2+} $, 反应7 d后DMP的降解效率显著提升到100%。此外, 过氧化物酶在DMP降解过程中起重要作用, 实验证明DMP的降解更多地由锰过氧化物酶(manganese peroxidase, MnP)驱动; 傅里叶红外光谱证实氨基、羧基、羟基等还原性官能团参与了DMP的去除。文中通过液相色谱-质谱(liquid chromatography-mass spectrometry, LC-MS)联用仪确定DMP的降解产物, 并讨论3种可能的降解途径。

关键词: 生物降解；塑化剂；白腐真菌；邻苯二甲酸二甲酯(DMP)；过氧化物酶

Funding

国家重点研发计划资助项目(2019YFC0408500); 安徽省科技重大专项资助项目(201903a07020009)和宿州市科技计划资助项目(2019056)

合肥工业大学学报(自然科学版)

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