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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Isolation, identification and pathogenicity analysis of kiwifruit bacterial canker pathogen

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猕猴桃细菌性溃疡病菌的分离鉴定与致病性分析

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Feb.2024, Vol.47 No.2: 257-262

DOI: 10.3969/j.issn.1003-5060.2024.02.018

Authors

WU Zhongqiu, NIU Xiangli, WANG Wenjing, LI Xuan, LIU Junfei, WU Jietao

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Kiwifruit canker is the main bacterial disease caused by Pseudomonas syringae pv. actinidiae (Psa). In this work, Psa strains were isolated and identified from diseased kiwifruit plants in different orchards for pathogenicity analysis. The results showed that six strains with P. syringae like morphological characteristics were obtained. After polymerase chain reaction (PCR) identification and sequence alignment, the isolates were identified as Psa. The inoculation of these strains in kiwifruit cultivar Hongyang exhibited their different pathogenic capabilities. ScYbH2, ScDjyH2 and ScYbH1 caused canker disease symptoms in inoculated plants, while ScMbJ1, ScDjyH3 and ScYaH2 did not possess serious pathogenicity. Moreover, the hypersensitivity reactions shown in tobacco leaves indicated different plant immunity activation elicited by the Psa isolates. In this study, isolated kiwifruit bacterial canker strains were obtained, and their phenotypic difference will provide a basis for further genetic analyses of pathogenic genes.

Keywords: kiwifruit; Pseudomonas syringae pv. actinidiae(Psa); pathogen isolation; pathogenicity; hypersensitivity reaction

作者信息

吴仲秋，牛向丽，汪雯静，李璇，刘骏菲，邬杰涛

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 溃疡病是猕猴桃主要细菌性病害，其病原为丁香假单胞菌猕猴桃致病变种（Pseudomonas syringae pv. actinidiae, Psa）。文章利用不同猕猴桃种植园的溃疡病发病植株进行 Psa 菌株分离、鉴定及致病性分析；结果显示，获得了 6 个具有丁香假单胞菌形态特征的菌株，经聚合酶链式反应（polymerase chain reaction，PCR）鉴定与序列同源比对，所分离菌株为 Psa，该菌株在“红阳”猕猴桃的接种检测中显示不同致病性，其中 ScYbH2、ScDjyH2、ScYbH1 接种植株出现溃疡病症状，而 ScMbJ1、ScDjyH3、ScYaH2 未表现显著致病性。烟草叶片超敏反应也显示它们具有不同的植物免疫系统激活能力。该研究获得了猕猴桃溃疡病分离菌株，其表型差异为后续的致病基因遗传分析奠定了基础。

关键词: 猕猴桃；丁香假单胞菌猕猴桃致病变种(Psa)；病原菌分离；致病性；超敏反应中图分类号：Q945.8 文献标志码：A 文章编号：1003-5060(2024)02-0257-06

Funding

国家自然科学基金资助项目（31671266）；合肥工业大学大学生创新创业训练计划资助项目（S202210359387）

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