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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Optimization of extraction process and antioxidant mechanism of Polygonatum cyrtonema Hua polysaccharides

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多花黄精多糖提取工艺优化及抗氧化机制研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), May.2024, Vol.47 No.5: 678-684

DOI: 10.3969/j.issn.1003-5060.2024.05.016

Authors

GUO Anjun, LI Xueying, LI Xiaowei, LUO Jianping, ZHA Xueqiang

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: In this paper, a response surface methodology was employed to optimize the extraction process of Polygonatum cyrtonema Hua polysaccharides (PCP) by hot water based on single factor test, considering the parameters of ratio of raw material to water, extraction temperature and extraction time. The PCP was isolated and purified to obtain water-washed polysaccharides. The results showed that the optimal extraction parameters were as follows: ratio of raw material to water of 1:25, extraction temperature of 85 $ ^{\circ} $C, and extraction time of 2.0 h. On this basis, the antioxidant mechanism of PCP was investigated using oxidized low-density lipoprotein(Ox-LDL)-treated macrophages. The MTT results indicated that PCP was not toxic to macrophages. Compared with the model group, PCP significantly increased the levels of total antioxidant capacity (T-AOC) and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and decreased the content of malondialdehyde (MDA), indicating that PCP could enhance antioxidant effects in Ox-LDL-induced macrophages. Furthermore, Western Blot experiments showed that PCP could also significantly upregulate the protein level of Nrf2 and down-regulate the protein expression of Keap1 in Ox-LDL-induced macrophages. These results revealed that PCP could regulate oxidative stress in macrophages induced by Ox-LDL, which might be related to the modulation of Keap1/Nrf2 signaling pathway. The study results may provide a theoretical basis for the further development and utilization of PCP.

Keywords: Polygonatum cyrtonema Hua polysaccharides(PCP); response surface methodology; macrophages; oxidative stress

作者信息

郭安君，李雪影，李晓炜，罗建平，查学强

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 文章以料液比、提取温度、提取时间为参数, 在单因素试验的基础上, 采用响应面法优化多花黄精多糖(Polygonatum cyrtonema Hua polysaccharides, PCP)的提取工艺, 并分离纯化得到水洗糖; 采用氧化低密度脂蛋白(oxidized low-density lipoprotein, Ox-LDL)诱导巨噬细胞氧化应激为实验模型, 研究PCP的抗氧化机制。结果显示, PCP的最佳提取工艺为: 料液比1:25, 提取温度85℃, 提取时间2.0 h。MTT实验结果表明, PCP对巨噬细胞不具有毒性; 与模型组相比, PCP显著提高了巨噬细胞的总抗氧化能力(total antioxidant capacity, T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的酶活力, 降低了丙二醛(MDA)的量, 表明PCP具有较强的抗氧化功能。Western Blot实验结果显示, PCP显著上调了Nrf2的蛋白水平, 抑制了Keap1的蛋白表达, 提示PCP发挥抗氧化作用可能与调控Keap1/Nrf2信号通路有关。研究结果为PCP的深入开发利用提供了一定的理论基础。

关键词: 多花黄精多糖(PCP)；响应面法；巨噬细胞；氧化应激

Funding

国家自然科学基金资助项目（32072176）

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