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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Functional study on the regulation of anthocyanin accumulation in pear peel by PyTPL2 gene

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梨 PyTPL2 基因调控果皮花青苷积累的功能研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Oct.2025, Vol.48 No.10: 1397-1400, 1410

DOI: 10.3969/j.issn.1003-5060.2025.10.015

Authors

AN Xin, YAO Gaifang, YAO Wenjie, LIU Zhi, HU Kangdi, ZHANG Hua

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Because of its rich anthocyanins and high nutritional value, red-skinned pear is favored by consumers and has become a hot spot for the development of China's characteristic pear industry. It has been reported that the binding of transcription factors with EAR motif to TOPLESS/TOPLESS-related(TPL/TPR) has a transcriptional inhibitory effect on the accumulation process of anthocyanins. In order to further investigate its function, this paper uses polymerase chain reaction (PCR) to clone the coding sequence (CDS) full-length and specific region silencing sequence of the PyTPL2 gene, which were connected to pSAK277 transient expression vector, and the recombinant plasmid was transferred into the expression strain GV3101, and then co-injected with the transcription factor PyMYB6 with EAR motif. It was found that PyMYB6 could inhibit the synthesis of anthocyanins when co-injected with PyTPL2, and PyMYB6 could promote the synthesis of anthocyanins when co-injected with PyTPL2-RNAi. The results provide a reference for further exploration of the role of pear PyTPL2 gene in pear peel anthocyanins.

Keywords: pear; anthocyanin; PyTPL2; homologous recombination; transient expression

作者信息

安欣，姚改芳，姚文杰，刘志，胡康棣，张华

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 红皮梨因富含花青苷且营养价值高，备受消费者青睐，成为我国特色梨产业发展的热点。据报道，具有EAR motif的转录因子与TOPLESS/TOPLESS-related（TPL/TPR）结合对花青苷积累过程具有转录抑制作用，为进一步研究其功能，文章利用聚合酶链式反应（polymerase chain reaction，PCR）克隆PyTPL2基因的编码序列（coding sequence，CDS）全长和特异区域沉默序列，连接至pSAK277瞬时表达载体，并将重组质粒转入表达菌株GV3101，然后与具有EAR motif的转录因子PyMYB6共同注射早酥梨。研究发现，PyMYB6与PyTPL2共同注射时可以抑制花青苷的合成，PyMYB6与PyTPL2-RNAi共同注射时可以促进花青苷的合成。该结果为进一步探究梨PyTPL2基因在梨果皮花青苷中的作用提供参考。

关键词: 梨；花青苷；PyTPL2；同源重组；瞬时表达

Funding

国家自然科学基金资助项目（32170315；32272682）

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