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J. Hefei Univ. Tech. (Nat. Sci.)

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

Competent Authorities: Ministry of Education of the People's Republic of China

Sponsor by: Hefei University of Technology

ISSN 1000-1646, CN 34-1001/N

Construction and function of VIGS vector of SlBLI2 gene in tomato

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番茄 SlBLI2 基因 VIGS 载体的构建及其功能研究

Journal Information

J. Hefei Univ. Tech. (Nat. Sci.), Oct.2025, Vol.48 No.10: 1383-1388

DOI: 10.3969/j.issn.1003-5060.2025.10.013

Authors

XIAO Yuwei, ZHANG Hua, WANG Wenjie, ZHAO Weisheng, YAO Gaifang, HU Kangdi

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: Tomato(Solanum lycopersicum) has high nutritional value and is popular among consumers. Currently, tomato has become one of the widely grown cash crops. The BLISTER(BLI) protein is a regulator of many stress responses, which promotes plant adaptation to the environment and plays an important role in regulating plant maturation. In this paper, the SlBLI2(Solyc12g097070.3) gene of tomato was used to analyze the sequence characteristics of the BLI family genes. By finding the specific region silencing sequence of the SlBLI2 gene, a tomato SlBLI2 virus-induced gene silencing(VIGS) vector was successfully constructed using homologous recombination technology. The tomato fruits were injected with different bacteria using gene silencing assay, and SlBLI2 was found to have a function in inhibiting fruit ripening, providing a reference for further investigation into the role of the tomato SlBLI2 gene in fruit ripening.

Keywords: tomato (Solanum lycopersicum); virus-induced gene silencing (VIGS) vector; SlBLI2 gene; homologous recombination; ripening and senescence

作者信息

肖钰薇，张华，汪文杰，赵维升，姚改芳，胡康棣

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 番茄(Solanum lycopersicum)具有较高的营养价值，深受消费者的喜爱，目前番茄已经成为广泛种植的经济作物之一。BLISTER(BLI)蛋白是许多应激反应的调节因子，可以促进植物对环境的适应性，在调控植物成熟方面发挥了重要作用。文章以番茄的SlBLI2(Solyc12g097070.3)基因为研究对象，对BLI家族的基因序列特征进行分析。通过SlBLI2基因的特异性区域沉默序列，利用同源重组技术成功构建了番茄StBLI2病毒诱导的基因沉默(virus-induced gene silencing, VIGS)载体。文章利用基因沉默实验对番茄果实注射不同杆菌，发现SlBLI2具有抑制果实成熟功能，为进一步探究番茄SlBLI2基因在果实成熟中的作用提供参考。

关键词: 番茄；VIGS载体；SlBLI2基因；同源重组；成熟衰老

Funding

国家自然科学基金资助项目（31970312；31970200；32170315；32272682）

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