Abstract: UV resistance locus 8(UVR8) encodes a photoreceptor protein, which is responsible for UV-B sensing and signal transduction, and is believed to be involved in the regulation of other physiological reactions. In this work, the fruits of UVR8 overexpression and gene-silenced plants were analyzed, and it showed that compared with the control group, the UVR8 overexpressed transgenic tomato (Solanum lycopersicum) fruits had a longer storage period, and the fruit hardness was significantly higher than that of the wild type. The catalase(CAT) and peroxidase(POD) activities of UVR8 transgenic tomato fruits were measured, and the results showed that the activities of CAT and POD in overexpressed tomato fruits were significantly higher than those in the wild type, which were 2.5 and 2.3 times higher than those in the wild type, respectively; while the activities of CAT and POD in gene-silenced tomato fruits were significantly lower than those in the wild type. The analysis of malondialdehyde(MDA), which is an indicator of lipid peroxidation, showed that the content of MDA per gram of overexpressed tomato fruits was reduced, which was only half of that of the wild type, and the content of MDA per gram of gene-silenced tomato fruits was significantly higher than that of the wild type. It could be inferred from the above results that the UVR8 gene played a role in prolonging the postharvest storage period of tomato fruits.
Keywords: tomato(Solanum lycopersicum); UV resistance locus 8(UVR8); storage period; enzyme activity; transgene
合肥工业大学学报自科版