合肥工业大学校徽

合肥工业大学学报自科版

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

主管：中华人民共和国教育部

主办：合肥工业大学

ISSN 1003-5060, CN 34-1083/N

丙二酸盐克罗诺杆菌 pspA 基因的干燥诱导及表达载体构建

Desiccation induction and expression construct of pspA gene from Cronobacter malonaticus

期刊信息

合肥工业大学（自然科学版），2023年3月，第46卷第3期：402-407

DOI: 10.3969/j.issn.1003-5060.2023.03.019

作者信息

汪馨，董晶晶，叶应旺

（合肥工业大学食品与生物工程学院，安徽合肥230601）

摘要和关键词

摘要: 噬菌体休克蛋白(phage shock protein, Psp)系统目前已被证明与包膜应激反应相关。为了探索干燥胁迫下丙二酸盐克罗诺杆菌(Cronobacter malonaticus)噬菌体休克蛋白A(PspA)的作用，文章通过实时荧光定量聚合酶链式反应(polymerase chain reaction, PCR)技术验证干燥胁迫可以诱导丙二酸盐克罗诺杆菌PspA编码基因pspA显著表达，提示Psp系统对克罗诺杆菌抗干燥胁迫具有一定的作用。该文成功克隆出丙二酸盐克罗诺杆菌pspA基因并构建pET28a-EGFP-PspA原核表达载体，诱导PspA的高效表达，可用于后续分析PspA在干燥胁迫下的功能及定位情况，以期对丙二酸盐克罗诺杆菌Psp系统的干燥胁迫机制展开进一步的研究和讨论。

关键词: 丙二酸盐克罗诺杆菌；pspA 基因；原核表达；干燥胁迫

Authors

WANG Xin, DONG Jingjing, YE Yingwang

(School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)

Abstract and Keywords

Abstract: The phage shock protein(Psp) system has been considered to be related to the envelope stress response(ESR). In this paper, the role of phage shock protein A(PspA) in Cronobacter malonaticus under desiccation stress was explored. Quantitative real-time polymerase chain reaction (PCR) result showed pspA encoded by PspA in C. malonaticus was significantly expressed by desiccation stress, suggesting that Psp system has a certain effect on the resistance of Cronobacter to desiccation stress. The pspA of C. malonaticus was successfully cloned and pET28a-EGFP-PspA expression construct was produced. Inducing the high-efficiency expression of PspA can be used to analyze the function and location of PspA under desiccation stress for carrying out further research and discussion on the desiccation tolerance mechanism of C. malonaticus Psp system.

Keywords: Cronobacter malonaticus; pspA gene; prokaryotic expression; desiccation stress

基金信息

国家自然科学基金资助项目（31972175）

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