Abstract: Polygonatum cyrtonema Hua, a common medicinal and edible homologous plant, belongs to the genus Polygonatum Mill. (Liliaceae), which can be processed by nine steaming and nine processing with rice wine. In this paper, firstly, the aqueous extracts of crude and wine-processed P. cyrtonema Hua were obtained by hot water extraction. After that, the aqueous extracts were precipitated by alcohol, and the supernatants were centrifuged, concentrated and dried to obtain the liposoluble part. The alcohol precipitates were then deproteinized, dialysed and dried to obtain the polysaccharide part. And significant difference was found in extraction rate between the two parts. Then the antioxidant activity of the aqueous extracts was evaluated in vitro, the total antioxidant capacity(T-AOC) and free radical scavenging ability of DPPH of the aqueous extract were significantly increased after nine steaming and nine processing. By measuring the indexes of T-AOC and DPPH, it was found that the antioxidant activity of the liposoluble and polysaccharide parts of wine-processed P. cyrtonema Hua aqueous extract was significantly higher than that of crude P. cyrtonema Hua aqueous extract. This paper is of significance to understand the pharmacodynamic basis and clinical application of P. cyrtonema Hua.
Keywords: aqueous extract of Polygonatum cyrtonema Hua; processing; liposoluble part; polysaccharide part; antioxidant activity in vitro