Abstract: WRKY transcription factors play important roles in plant growth and development, secondary metabolite synthesis, and response to biotic and abiotic stresses. In order to investigate the function and mechanism of action of WRKY3 in plant adversity response, this study analyzed the structural domains and evolutionary trees of WRKY3 in different species by bioinformatic method and found that WRKY3 was highly conserved. TomExpress database was used to analyze WRKY3 expression level in different tissues of tomato. The result showed that WRKY3 was expressed in various tissues, and the highest in the fruit at the red-fruit stage. The CRISPR knockdown vector of WRKY3 gene was constructed. WRKY3 transgenic tomato plants were obtained by using Agrobacterium-mediated transformation and identified by polymerase chain reaction (PCR) and sequencing. In this study, the stable genetic knockout plants of tomato WRKY3 was obtained, which lays the foundation for the investigation of the function of WRKY3 in plant growth and development and fruit ripening under adversity stress.
Keywords: tomato(Solanum lycopersicum); WRKY3 gene; gene editing; CRISPR/Cas9 technology; vector construction
合肥工业大学学报自科版