大球盖菇多糖的体外模拟消化研究-学报杂志社

期刊信息

合肥工业大学（自然科学版），2023年6月，第46卷第6期：859-864

DOI: 10.3969/j.issn.1003-5060.2023.06.022

作者信息

金明枝 $ ^{1} $，黄千里 $ ^{1} $，陈辉 $ ^{2} $，叶明 $ ^{1} $

(1. 合肥工业大学食品与生物工程学院, 安徽合肥 230601; 2. 上海市农业科学院食用菌研究所, 上海 201403)

摘要和关键词

摘要: 文章从大球盖菇子实体中提取分离得到均一组分多糖(SP-1a)，采用体外模拟唾液、胃液和肠液模型，并结合理化性质与体外抗氧化活性，研究SP-1a在体外模拟消化体系中的变化。结果表明：唾液对SP-1a无显著影响；而在模拟胃液和肠液中，随着还原糖质量浓度的不断增加，SP-1a的分子量逐渐减小，其表面形态由大块片状变为网状聚集体，并且在高温下显现出不同的热解反应。此外体外抗氧化实验结果表明，SP-1a胃消化产物对·OH具有较强的清除能力，肠消化产物对DDPH自由基具有较强的清除能力及还原力。因此，SP-1a可在模拟胃肠体系中被消化降解，且其胃肠消化产物具有较强的抗氧化活性。

关键词: 大球盖菇；多糖；体外消化；理化性质；抗氧化活性

Authors

JIN Mingzhi $ ^{1} $, HUANG Qianli $ ^{1} $, CHEN Hui $ ^{2} $, YE Ming $ ^{1} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

Abstract and Keywords

Abstract: A homopolysaccharide(SP-1a) was extracted from the fruit bodies of Stropharia rugosoannulata. By simulating the digestion of human gastrointestinal tract in vitro and combining physicochemical properties with in vitro antioxidant activity, the changes of SP-1a in the simulated digestive system were explored. The results showed that saliva had no significant effect on SP-1a, but in gastrointestinal digestive system, with the increasing content of reducing sugars, the molecular weight of SP-1a gradually decreased, the surface morphology of SP-1a changed from large flakes to reticular aggregates, and it showed different pyrolysis reactions at high temperature. The results of in vitro antioxidant experiments showed that SP-1a gastric digestive products had strong $ \cdot $OH scavenging ability, and intestinal digestive products had strong DPPH scavenging ability and reducing power. Therefore, SP-1a can be digested in the simulated gastrointestinal system, and its gastrointestinal digestive products have strong antioxidant activity.

Keywords: Stropharia rugosoannulata; polysaccharide; digestion in vitro; physicochemical property; antioxidant activity

基金信息

上海市科技兴农资助项目（2019-02-08-00-12-F01132）；合肥工业大学产学研校企合作资助项目（W2019JSKF0082）

合肥工业大学学报(自然科学版)

导航菜单

大球盖菇多糖的体外模拟消化研究