一株耐酸曲霉的优化培养及其对 $ Cu^{2+} $ 的去除作用-学报杂志社

期刊信息

合肥工业大学（自然科学版），2024年5月，第47卷第5期：667-672

DOI: 10.3969/j.issn.1003-5060.2024.05.014

作者信息

贾亚文 $ ^{1} $，王进 $ ^{1} $，王绍平 $ ^{2} $，王广成 $ ^{2} $，李伟 $ ^{2} $，岳正波 $ ^{1} $

(1. 合肥工业大学资源与环境工程学院，安徽合肥 230009；2. 安徽马钢矿业资源集团南山矿业有限公司，安徽马鞍山 243000)

摘要和关键词

摘要: 生物修复是处理重金属污染的重要手段之一,真菌在生物修复中具有良好的应用前景。文章从安徽省马鞍山市某矿山废水中分离出一株丝状真菌并研究其对 $ Cu^{2+} $的去除作用。经形态学及18SrDNA序列分析鉴定为曲霉,命名Aspergillus sp. MF1。研究表明:在pH值为3.5条件下,碳源为葡萄糖、氮源为酵母、碳氮比为10.0时有最大生物量。菌体对 $ Cu^{2+} $最大耐受质量比为770.18 mg/g,并能有效地去除 $ Cu^{2+} $。 $ \rho_{0}(Cu^{2+}) $为50、100 mg/L时,Aspergillus sp. MF1对 $ Cu^{2+} $的去除率分别可以达到78.49%和64.63%,且 $ \omega_{\mathrm{max}}(\mathrm{Cu}^{2+})=123.99\ \mathrm{mg/g} $。在Aspergillus sp. MF1抗 $ Cu^{2+} $胁迫的生理响应过程中,蛋白质的作用随着 $ \rho(\mathrm{Cu}^{2+}) $增大而增加,随时间的增长而减小。菌株Aspergillus sp. MF1具有在强酸性环境下去除 $ Cu^{2+} $的能力,该研究可为真菌在极端环境下治理 $ Cu^{2+} $污染提供一定的参考。

关键词: 丝状真菌；生长条件；重金属；生长胁迫

Authors

JIA Yawen $ ^{1} $, WANG Jin $ ^{1} $, WANG Shaoping $ ^{2} $, WANG Guangcheng $ ^{2} $, LI Wei $ ^{2} $, YUE Zhengbo $ ^{1} $

(1, School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China; 2, Nanshan Mining Co., Ltd., Anhui Masteel Mining Resources Group, Ma'anshan 243000, China)

Abstract and Keywords

Abstract: Bioremediation is one of the important means to deal with heavy metal pollution, and fungi have a good application prospect in bioremediation. A filamentous fungus was isolated from wastewater from a mine in Ma'anshan City, Anhui Province and its effect on the removal of $ Cu^{2+} $ was studied. It was identified as Aspergillus sp. MF1 by morphological analysis and 18SrDNA sequence analysis. The results showed that the maximum biomass was obtained when the pH value was 3.5, the carbon source was glucose, the nitrogen source was yeast, and the carbon to nitrogen ratio was 10.0. The maximum tolerance mass rate of $ Cu^{2+} $ was 770.18 mg/g, and $ Cu^{2+} $ could be effectively removed. When $ \rho_{0}\left(\mathrm{Cu}^{2+}\right) $ was 50 and 100 mg/L, the $ Cu^{2+} $ removal rates of Aspergillus sp. MF1 could reach 78.49% and 64.63%, respectively, and $ \omega_{\mathrm{max}}\left(\mathrm{Cu}^{2+}\right) $ was 123.99 mg/g. In the physiological response of Aspergillus sp. MF1 to $ Cu^{2+} $ stress, the role of proteins increased with the increase of $ \rho\left(\mathrm{Cu}^{2+}\right) $ and decreased with the increase of time. Aspergillus sp. MF1 has the ability to remove $ Cu^{2+} $ in a strongly acidic environment, and this study can provide reference for the treatment of $ Cu^{2+} $ pollution by fungi in extreme environments.

Keywords: filamentous fungi; growth conditions; heavy metals; growth stress

基金信息

国家自然科学基金区域创新发展联合基金（安徽）资助项目（U20A20325）

合肥工业大学学报(自然科学版)

导航菜单

一株耐酸曲霉的优化培养及其对 $ Cu^{2+} $ 的去除作用