白玉菇抗肿瘤活性多糖 WHB3 的结构表征-学报杂志社

期刊信息

合肥工业大学（自然科学版），2024年8月，第47卷第8期：1106-1111，1117

DOI: 10.3969/j.issn.1003-5060.2024.08.015

作者信息

李孟欣 $ ^{1,2} $，张娅娣 $ ^{1,3} $，刘咏 $ ^{1} $，王军辉 $ ^{1,2} $

（1. 合肥工业大学食品与生物工程学院，安徽合肥 230601；2. 合肥工业大学农产品生物化工教育部工程研究中心，安徽合肥 230601；3. 安徽省粮油产品质量监督检测站，安徽合肥 230031）

摘要和关键词

摘要: 文章采用热水浸提及 DEAE-Cellulose 离子交换层析从白玉菇 (White Hypsizygus marmoreus) 子实体中分离纯化出均一多糖组分 WHB3。糖醛酸测定表明 WHB3 不含糖醛酸；单糖组成分析表明 WHB3 由葡萄糖组成；相对分子质量测定结果为 $ 2.3 \times 10^{5} $ Da；结构分析结果表明，WHB3 主链由 $ (1 \rightarrow 6) $- $ \beta $-D-Glcp 组成，其中部分 $ (1 \rightarrow 6) $- $ \beta $-D-Glcp 的 O-3 位连有支链 1- $ \alpha $-D-Glcp。通过体外肝癌细胞 HepG2 抗肿瘤实验模型考察白玉菇多糖 WHB3 及其部分酸水解产物 WHB3-S 的抗肿瘤活性，结果表明 WHB3 和 WHB3-S 对肝癌细胞 HepG2 的增殖具有显著的抑制作用，且与多糖质量浓度呈正相关，多糖质量浓度达到 $ 500 \mu g/mL $ 时，抑制率分别达到 38.18%、35.88%。构效关系分析表明，与 WHB3 相比，WHB3-S 在各质量浓度下的抑制率明显较低，说明相对分子质量的减小及支链的缺失对 WHB3 的抗肿瘤活性有显著的影响，降低了 WHB3 的肿瘤抑制活性。研究结果为揭示白玉菇多糖化学结构与生物活性之间的潜在联系提供更多依据。

关键词: 白玉菇；多糖；结构表征；抗肿瘤活性；构效关系

Authors

LI Mengxin $ ^{1,2} $, ZHANG Yadi $ ^{1,3} $, LIU Yong $ ^{1} $, WANG Junhui $ ^{1,2} $

(1. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; 2. Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology, Hefei 230601, China; 3. Anhui Grain and Oil Products Quality Supervision and Testing Station, Hefei 230031, China)

Abstract and Keywords

Abstract: In this paper, the crude polysaccharide was isolated from the fruiting bodies of white Hyp-sizygus marmoreus by water extraction at boiling temperature. After purification by DEAE-Cellulose ion exchange chromatography, the homogenous polysaccharide fraction WHB3 was obtained. The results of uronic acid determination showed that WHB3 contained no uronic acid. Monosaccharide composition analysis showed that WHB3 contained only glucose and its relative molecular mass was determined to be $ 2.3 \times 10^{6} $ Da. Structural characterization results indicated that the main chain of WHB3 was consisted of $ (1 \rightarrow 6) $- $ \beta $-D-Glcp, part of which was connected with branched 1- $ \alpha $-D-Glcp at the O-3 position. The anti-tumor activity of WHB3 and its hydrolysate WHB3-S on HepG2 cells was investigated in vitro by establishing hepatocellular carcinoma cell model. The results showed that the inhibitory effects of WHB3 and WHB3-S on HepG2 cells were positively correlated with the polysaccharide mass concentration. The structure-activity relationship analysis showed that the inhibitory rates of WHB3 and WHB3-S on HepG2 cells were 38.18% and 35.88% when the polysaccharide mass concentration was 100 mg/g.

Keywords: white Hypsizygus marmoreus; polysaccharide; structural characterization; anti-tumor activity; structure-activity relationship

基金信息

安徽省科技重大专项资助项目（201903a06020024）

合肥工业大学学报(自然科学版)

导航菜单

白玉菇抗肿瘤活性多糖 WHB3 的结构表征