Abstract: Anthocyanin is a common pigment in fruit, and its role in fruit response to pathogen infection is unclear. In this paper, the wild type(WT) strains of Aspergillus niger(A. niger) and the related strains with deletion of antioxidant enzymes $ \Delta cpeA $, $ \Delta sodB $ and $ \Delta sodC $ were used to infect Dangshan pear and Starkrimson pear. Anthocyanin-rich Starkrimson pears were found to be highly resistant to A. niger. Therefore, the anthocyanins of Starkrimson pear were extracted, and Escherichia coli(E. coli) and A. niger were treated with 0, 5% and 10% anthocyanins, respectively, and it was found that 5% and 10% anthocyanins showed different inhibitory effects on both E. coli and A. niger. The Starkrimson pears were infected by A. niger, and the expression changes of anthocyanin-related genes in the infected lesions of pears were analyzed by RT-qPCR. The expression levels of PyMYB10, PyMYB114, PybHLH3, PyDFR, PyANS and PyUFGT were up-regulated, indicating that pear fruit may inhibit the infection of the postharvest pathogenic fungus A. niger by up-regulating the anthocyanin synthesis pathway.
Keywords: pear; anthocyanidin; bacteriostasis; Aspergillus niger(A. niger); Escherichia coli(E. coli)
合肥工业大学学报自科版