Abstract: The MYB family of proteins is a large and functionally abundant class of transcription factors present in all eukaryotes, and MYB proteins are mainly involved in morphogenesis, metabolism, and abiotic stress in plants. In this study, an R1-MYB transcription factor with EAR transcription inhibitor, SlMYBL, was found in tomato through National Center for Biotechnology Information (NCBI) database. The full-length cDNA of the SlMYBL gene was obtained by homologous cloning. SlMYBL gene is expressed in all tissues of tomato, and its expression in fruit is relatively high, followed by root and leaf. Studies have shown that R1-MYB transcription factors can participate in adversity response of plants. In order to understand whether SlMYBL responds to adversity response, the transcription level of SlMYBL gene in tomato materials under different stress treatment was quantitatively analyzed. It was found that the transcription level of SlMYBL gene decreased significantly under salt stress, but there was no significant difference in mannitol stress, suggesting that SlMYBL gene may be involved in the response of tomato plants to salt stress. In order to study the molecular mechanism of SlMYBL gene, the CRISPR expression vector of SlMYBL gene was constructed, and the CRISPR knockout transgenic tomato of SlMYBL gene was obtained. This study lays a theoretical foundation for discovering the role of SlMYBL gene in plant growth and development.
Keywords: tomato(Solanum lycopersicum); SlMYBL gene; adversity stress; CRISPR/Cas9 technology; vec
合肥工业大学学报自科版