合肥工业大学学报自科版

合肥工业大学学报(自然科学版)

JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY (NATURAL SCIENCE)

主管：中华人民共和国教育部

主办：合肥工业大学

ISSN 1003-5060, CN 34-1083/N

单宁酸改性鱼皮明胶-透明质酸复合微球的制备及性质研究

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Preparation and properties of tannic acid modified fish skin gelatin-hyaluronic acid composite microspheres

期刊信息

合肥工业大学（自然科学版），2025年8月，第48卷第8期：1098-1105，1111

DOI: 10.3969/j.issn.1003-5060.2025.08.014

作者信息

刘世龙 $ ^{1,2} $，王彦波 $ ^{3} $，张龙涛 $ ^{1,2} $，侯国华 $ ^{1,2} $，郑宝东 $ ^{1,2} $

（1. 福建农林大学闽台特色海洋食品加工及营养健康教育部工程研究中心，福建福州 350002；2. 福建农林大学食品科学学院，福建福州 350002；3. 北京工商大学食品与健康学院，北京 100048）

摘要和关键词

摘要: 文章以大黄鱼鱼皮中提取的鱼皮明胶（fish skin gelatin，FSG）为主体材料，通过乳液模板法制备鱼皮明胶-透明质酸（fish skin gelatin-hyaluronic acid，FSG-HA）复合微球，再利用单宁酸（tannic acid，TA）对复合微球混合交联，构建具有高抗氧化及抗菌活性的单宁酸改性鱼皮明胶-透明质酸（FSG-HA/TA）复合微球。采用动态光散射仪、透射电子显微镜（transmission electron microscope，TEM）、激光扫描共聚焦显微镜（confocal laser scanning microscope，CLSM）、傅里叶变换红外光谱仪（Fourier transform infrared spectrometer，FTIR）和紫外-可见分光光度计等表征FSG-HA和FSG-HA/TA的微观形貌和结构，测定其抗氧化及抗菌活性。结果表明：FSG-HA和FSG-HA/TA均表现出球形结构，单宁酸的交联改性降低了FSG-HA的粒径分布，增强了鱼皮明胶与透明质酸之间的氢键作用，使微球结构更紧密且均匀；FSG-HA和FSG-HA/TA对DPPH自由基和ABTS阳离子自由基的清除率具有质量浓度依赖性，清除率最高分别达93.75%和67.63%。此外，改性后FSG-HA/TA对金黄色葡萄球菌和大肠杆菌的抑制效果显著，尤其是对金黄色葡萄球菌的最高抑菌率达95%。因此，FSG-HA/TA具有较强的抗氧化和抗菌性能，作为食品保鲜和生物医药领域的功能性材料具有巨大的应用潜力。

关键词: 大黄鱼；鱼皮明胶(FSG)；复合微球；抗氧化；抗菌

Authors

LIU Shilong $ ^{1,2} $, WANG Yanbo $ ^{3} $, ZHANG Longtao $ ^{1,2} $, HOU Guohua $ ^{1,2} $, ZHENG Baodong $ ^{1,2} $

(1. Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)

Abstract and Keywords

Abstract: Fish skin gelatin (FSG) extracted from Pseudosciaena crocea skin was used as the main material. Fish skin gelatin-hyaluronic acid (FSG-HA) composite microspheres were prepared by the emulsion template method. Tannic acid (TA) was used to cross-link the microspheres to construct TA-modified fish skin gelatin-hyaluronic acid (FSG-HA/TA) composite microspheres with high antioxidant and antibacterial activities. Dynamic light scattering, transmission electron microscope (TEM), confocal laser scanning microscope (CLSM), Fourier transform infrared spectrometer (FTIR) and ultraviolet-visible absorption spectroscopy were used to characterize the microscopic morphology and structure of FSG-HA and FSG-HA/TA, and their antioxidant and antibacterial activities were determined. mined. The results indicated that both FSG-HA and FSG-HA/TA exhibited spherical structures. The cross-linking modification of TA reduced the particle size distribution of FSG-HA microspheres, enhanced the hydrogen bonding between FSG and HA, and made the microsphere structure more compact and uniform. The scavenging rates of FSG-HA and FSG-HA/TA against DPPH radicals and ABTS cationic radicals were concentration-dependent, with the scavenging rates reaching up to 93.75% and 67.63%, respectively. In addition, the modified FSG-HA/TA had significant inhibitory effects on Staphylococcus aureus and Escherichia coli, with the inhibition rate against Staphylococcus aureus reaching up to 95%. In summary, FSG-HA/TA composite microspheres have strong antioxidant and antibacterial properties and exhibit significant potential as functional materials in the fields of food preservation and biomedicine.

Keywords: Pseudosciaena crocea; fish skin gelatin(FSG); composite microspheres; antioxidant; antibacterial

基金信息

国家重点研发计划资助项目（2022YFD2101305）

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