AHLs 强化 SBR 应对高盐胁迫硝化过程的研究

文章通过在序批式反应器（sequencing batch reactor，SBR）中添加外源性N-酰基-高丝氨酸内酯（AHLs）以促进体系内群体感应现象的发生，系统探究了不同碳链长度（C4-L-HSL、C6-L-HSL、C8-L-HSL）和不同取代基结构（C6-L-HSL、C6-DL-HSL、3-oxo-C6-HSL）的AHLs对SBR系统应对高盐度胁迫能力的影响及污泥性质变化。结果表明：相较于2%盐度，5%、8%盐度胁迫的前10 d， $ NH_{4}^{+}-N $的平均去除率分别下降了6.06%、18.32%，且出水中有大量 $ NO_{2}^{-}-N $的积累；在所有组别中，添加3-oxo-C6-HSL的实验组在应对盐度胁迫上表现最佳，周期内 $ NO_{2}^{-}-N $积累峰值在5%盐度、8%盐度分别低于对照组39.54%、58.09%， $ NH_{4}^{+}-N $去除率提升和 $ NO_{2}^{-}-N $积累改善最为明显。此外，对污泥胞外聚合物（extracellular polymeric substances，EPS）的提取分析发现，实验组的蛋白和多糖质量浓度分别提高了14.4%~38.4%和26.0%~41.0%。EPS的 $ UV_{254} $数据表明，实验组中大分子有机物及芳香族化合物质量浓度较高，说明其代谢途径更为丰富，产物也更为复杂。微生物群落分析表明变形杆菌门（Proteobacteria）是该系统中主要的优势菌门。

In this paper, exogenous N-acyl-homoserine lactones(AHLs) were added to a sequencing batch reactor(SBR) to promote quorum sensing(QS). The effects of AHLs with different carbon chain lengths(C4-L-HSL, C6-L-HSL, and C8-L-HSL) and different substituent structures(C6-L-HSL, C6-DL-HSL, and 3-oxo-C6-HSL) on the SBR system's ability to cope with high salinity stress and the changes in sludge properties were systematically investigated. Experiments showed that under 5% and 8% salinity stress for the first ten days, compared to 2% salinity, the average $ NH_{4}^{+}-N $ removal rate decreased by 6.06% and 18.32%, respectively, with significant $ NO_{2}^{-}-N $ accumulation in the effluent. Among all groups, the group with added 3-oxo-C6-HSL best alleviated salinity stress, reducing the $ NO_{2}^{-}-N $ accumulation peak by 39.54% and 58.09% under 5% and 8% salinity, respectively, and showed remarkable improvement in $ NH_{4}^{+}-N $ removal and $ NO_{2}^{-}-N $ accumulation. Moreover, the analysis of extracellular polymeric substances(EPS) showed that the protein and polysaccharide mass concentrations in the experimental group were increased by 14.4%-38.4% and 26.0%-41.0%, respectively. The $ UV_{254} $ data of EPS showed that the content of large molecular organic matter and aromatic compounds in the experimental group was higher, indicating that the metabolic pathways in the experimental group were more diverse and the products were more complex. Analysis of the microbial community indicated that Proteobacteria was the dominant phylum in the system. community indicated that Proteobacteria was the dominant phylum in the system.