有机碳源对小球衣藻生长和聚集的影响

由于碳源作为主要营养物质对于微藻的培养过程有着重要影响，文章以小球衣藻(Chlamydomonas microsphaera)为研究对象，探究在葡萄糖和乙酸钠2种有机碳源影响下，微藻生长和聚集的变化。研究结果表明：一次性投加化学需氧量(chemical oxygen demand, COD)为$100\mathrm{mg / L}$ 的乙酸钠时，菌藻细胞数量比为3：1的菌藻体系中有最大微藻聚集率 $63\%$ ，是纯藻对照组的2.2倍，是无碳源实验组的1.8倍；乙酸钠和葡萄糖对于微藻的聚集和生长均有促进作用，且乙酸钠促进效果优于葡萄糖，但是随着其投加量增加，促进微藻聚集和生长的效果下降，甚至出现抑制生长的情况；此外，相较于一次性投加碳源，分批式投加碳源更有利于微藻生长。

Considering the significant impact of carbon source as the main nutrient on the cultivation process of microalgae, this study focuses on Chlamydomonas microsphaera and explores the changes in microalgae growth and aggregation under the influence of two organic carbon sources, glucose and sodium acetate. Research has found that when sodium acetate with a chemical oxygen demand(COD) of$100\mathrm{mg / L}$ is added at once, the maximum microalgae aggregation rate is $63\%$ in a bacterial-algal system with a ratio of bacteria to algae of $3:1$ , which is 2.2 times that of the pure algae control group and 1.8 times that of the carbon free control group. Both sodium acetate and glucose have a promoting effect on the aggregation and growth of microalgae, and sodium acetate has a better promoting effect than glucose. However, as the dosage increases, the effect of promoting microalgae aggregation and growth decreases, and even growth is inhibited. In addition, compared to one-time addition of carbon sources, batch addition of carbon sources is more conducive to the growth of microalgae.