拟南芥 MED18 启动子的克隆及其遗传转化分析

近年来，由碱性碳酸盐土壤诱发植物缺铁黄化的现象十分普遍，其严重制约着该类土壤上的作物生产力。MED18是植物中介体复合物的一个多功能亚基，参与调控植物生长发育和抗逆境胁迫等多种复杂的生理过程。文章以拟南芥为研究材料，利用聚合酶链式反应(polymerase chain reaction, PCR)扩增MED18基因启动子片段，通过同源重组方法成功构建出ProMED18:GUS融合表达载体；采用浸花法将ProMED18:GUS载体转入拟南芥植株中，并通过分子鉴定筛选出ProMED18:GUS转基因植株。研究结果为探究缺铁胁迫下MED18基因的功能提供了重要的参考依据。

In recent years, the phenomenon of plant iron deficiency and yellowing induced by alkaline carbonate soil has become increasingly prevalent, which seriously restricts crop productivity in this type of soil. Mediator18(MED18) is a multifunctional subunit of plant mediator complexes, involved in regulating various complex physiological processes such as plant growth and development and resistance to stress. This paper used Arabidopsis thaliana as the research material, amplified the MED18 gene promoter fragment using polymerase chain reaction(PCR), and successfully constructed the ProMED18:GUS fusion expression vector through homologous recombination method. The ProMED18:GUS vector was transferred into Arabidopsis thaliana plants using the floral dip method, and the ProMED18:GUS transgenic plants were screened through molecular identification, providing important reference for exploring the function of the MED18 gene under iron deficiency stress.