大豆中磷脂酶D基因家族生物学功能研究

为探究大豆中磷脂酶D(PLD)家族的生物学功能，文章利用染色体定位分析、基因结构分析、蛋白理化性质分析、核定位序列(nuclear localization sequence, NLS)、亚细胞定位分析预测、蛋白基序分析、蛋白结构域分析、蛋白二级和三级结构预测等生物信息学手段对大豆中5个亚型共21个PLD基因家族进行分析，发现在同一亚类中基因与蛋白结构更为保守，其结构与性质更为接近。通过对大豆中PLD基因表达水平进行分析，发现PLD基因可能在大豆种子发育、豆荚发育、开花、根和叶片生长方面发挥作用。研究结果为深入分析PLD基因家族在大豆中的生物学功能提供了参考。

In order to investigate the biological functions of phospholipase D(PLD) family in soybean, this paper utilized chromosomal localization analysis, gene structure analysis, protein physicochemical property analysis, nuclear localization sequence (NLS), subcellular localization prediction, protein motif analysis, protein structural domain analysis, and protein secondary and tertiary structure prediction for a total of twenty-one PLD gene families of five isoforms in soybean, and found that the genes and proteins were more conserved with the same subclasses, and their structures and properties were more similar. By performing an analysis of PLD gene expression levels in soybean, it was found that PLD genes might play a role in soybean seed and pod development, flowering, and root and leaf growth. The findings of the paper provide a reference for an in-depth study of the biological functions of PLD gene family in soybean.