硒酵母不同组分对 LLC 荷瘤小鼠的抗肿瘤作用

文章以 Lewis 肺癌（Lewis lung carcinoma，LLC）细胞建立荷瘤小鼠模型，比较硒酵母（selenium-enriched yeast）中的硒酵母蛋白、葡聚糖和甘露聚糖组分的抗肿瘤作用，明确硒酵母抗肿瘤的最佳活性组分，并分析其抗肿瘤作用机制。结果表明：硒酵母蛋白、葡聚糖和甘露聚糖均能不同程度地抑制荷瘤小鼠肿瘤生长，抑瘤率分别为 36.08%、18.62%、12.75%；3 个组分均能显著下调 B 淋巴细胞瘤-2（Bcl-2）、血管内皮生长因子（vascular endothelial growth factor，VEGF）、CD34 和白介素-10（IL-10）mRNA 的表达，显著上调 Bax、Casepase-3 和肿瘤坏死因子 $ \alpha $（tumor necrosis factor- $ \alpha $，TNF- $ \alpha $）mRNA 的表达，其中硒酵母蛋白的作用最强。说明硒酵母蛋白、葡聚糖和甘露聚糖均可通过调控肿瘤血管生成、免疫和细胞凋亡发挥抗肿瘤作用，其中硒酵母蛋白的抗肿瘤作用最强，是硒酵母抗肿瘤的最佳活性组分。

This paper compared the anti-tumor effects of different components of selenium-enriched yeast (selenium-enriched yeast protein, glucan and mannan) by using Lewis lung carcinoma (LLC) tumor-bearing mice model, identified the best active component of selenium-enriched yeast against tumors, and clarified its anti-tumor mechanism. The results showed that the tumor growth of LLC tumor-bearing mice could be inhibited by selenium-enriched yeast protein, glucan and mannan, and their inhibition rates were 36.08%, 18.62% and 12.75%, respectively. The mRNA expression of B-cell lymphoma-2(Bcl-2), vascular endothelial growth factor(VEGF), CD34 and interleukin-10(IL-10) could be significantly decreased by selenium-enriched yeast protein, glucan and mannan, while the mRNA expression of Bax, Caspase-3 and tumor necrosis factor-α (TNF-α) could be significantly increased. Selenium-enriched yeast protein exhibited the strongest regulative effect on the expression of these mRNAs. It showed that selenium-enriched yeast protein, glucan and mannan could inhibit the tumor growth of LLC tumor-bearing mice by regulating tumor angiogenesis, immunity and apoptosis, among which selenium-enriched yeast protein exhibited the strongest anti-tumor effect and was the best active component of selenium-enriched yeast against tumors.