植物激素 IAA 和维生素 $ B_{12} $对小球衣藻聚集和生长的影响

为了有效培养和收集微藻，文章以小球衣藻（Chlamydomonas microsphaera）为研究对象，研究生物收集法中植物生长激素吲哚乙酸（indole-3-acetic acid，IAA）和维生素 $ B_{12} $对于微藻生长与聚集的影响。结果表明：IAA和 $ B_{12} $对于微藻的聚集均有促进效果，且IAA和 $ B_{12} $质量浓度越高，促进效果越好，但是会抑制微藻的生长，此外，微藻在菌藻体系中的聚集率优于纯藻体系，但是微藻细胞数量却低于纯藻体系；在IAA初始质量浓度为0.1 mg/L、菌藻细胞数量比为3：1的菌藻共培养体系中有最大微藻聚集率39%。该研究可为有效培养和收集微藻提供一定理论基础和参数依据。

In order to effectively cultivate and collect microalgae, this paper focused on Chlamydomonas microsphaera and studied the effects of plant growth hormone indole-3-acetic acid (IAA) and vitamin $ B_{12} $ on microalgae growth and aggregation in biological collection methods. The results showed that both IAA and $ B_{12} $ had a promoting effect on the aggregation of microalgae, and the higher the concentration of IAA and $ B_{12} $, the better the promoting effect, but it would inhibit the growth of microalgae. In addition, it was found that the aggregation rate of microalgae in the bacterial-algal system was higher than that in the pure algal system, but the biomass was lower than that in the pure algal system. In a bacterial-algal co-culture system with an initial IAA concentration of 0.1 mg/L and a ratio of bacteria to algae of 3:1, the maximum microalgae aggregation rate was 39%. This study provides a theoretical basis and parameter reference for the effective cultivation and collection of microalgae.