独立 C 线改进的试纸条快速稳定检测沙丁胺醇

沙丁胺醇作为瘦肉精的一种, 最终会通过食物链对人体造成危害, 因此, 需要一种便捷、快速的方法用于现场检测。文章在传统胶体金免疫侧向层析的基础上提出一种独立 C 线的检测方法, 该方法引入独立 C 线抗原和独立 C 线抗体, 避免了传统试纸条中 C 线、T 线之间存在竞争的情况。对该试纸条进行优化后, 与传统试纸条体系相比, 稳定性更高。检测结果表明, 该试纸条对沙丁胺醇的检测限为 $ 5.0 \mu g/L $, 且仅需 5 min 即可完成检测, 在食品安全监测应用中具有可靠、快速现场检测沙丁胺醇的潜力。

Salbutamol, a form of lean meat powder, can eventually harm people through the food chain. Therefore, a convenient and rapid method is needed for field detection. This paper presents an independent C line method based on traditional colloidal gold immunolateral chromatography, which introduces independent C line antigen and independent C line antibody. The competition between C and T lines in the traditional test strips is avoided. Compared with the traditional test strip system, the stability of the optimized test strip is higher. The detection limit of salbutamol on the test strip is $ 5.0 \mu g/L $, and the method only takes 5 min, which has the potential for reliable and rapid field detection of salbutamol in food safety monitoring applications.