盐酸小檗碱碳点的合成及抗菌性能研究

文章以盐酸小檗碱为原料, 通过一步水热法合成盐酸小檗碱碳点(berberine hydrochloride carbon dots, BCDs)。利用透射电子显微镜(transmission electron microscope, TEM)、傅里叶变换红外光谱仪(Fourier transform infrared spectroscopy, FTIR)、紫外-可见分光光度计和荧光分光光度计对BCDs的形貌、结构和光学性质进行表征; 通过细菌生长曲线和稀释涂布平板法, 分别以食源性革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌为模式菌种, 研究BCDs的抗菌性能。结果表明: 制备的BCDs粒径小、分散性好, 且具有荧光性质; BCDs相较于盐酸小檗碱, 具有更强的抗菌能力, 且对革兰氏阳性菌的抗菌效果优于对革兰氏阴性菌的; 当BCDs质量浓度分别为40、320 $ \mu $g/mL时, 对金黄色葡萄球菌和大肠杆菌的抑制效果最好。

Berberine hydrochloride carbon dots (BCDs) were synthesized by a one-step hydrothermal method using berberine hydrochloride (Ber) as the raw material. The morphology, structure, and optical properties of BCDs were characterized by transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible (UV-vis) spectroscopy, and fluorescence spectroscopy. The antibacterial properties were studied by bacterial growth curves and spread plate method using foodborne Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) as model strains. The results showed that BCDs have a small particle size, good dispersion, and fluorescence properties. BCDs have a stronger antibacterial ability than the raw materials, and the antibacterial effect against Gram-positive bacteria was better than that against Gram-negative bacteria. When BCDs concentration was $ 40 \mu g/mL $ and $ 320 \mu g/mL $, respectively, the inhibition effect against S. aureus and E. coli was the best.