白介素-1β基因敲除对小鼠行为的影响

白介素-1β(interleukin-1β, IL-1β)是脑内一种常见的促炎因子, 在生理水平下由小胶质细胞和神经元释放并维持在较低表达量。文章选用成年小鼠为实验对象, 采用不同的行为学范式研究 IL-1β 基因敲除对小鼠行为的影响。研究发现, IL-1β 基因敲除不会影响小鼠的基本运动能力, 但显著减少了小鼠进入 Y 迷宫新臂和新物体识别测试中嗅探新物体的时间和次数, 增加了小鼠在悬尾以及强迫游泳测试中的静止时间。结果表明敲除 IL-1β 基因降低了小鼠的学习记忆能力, 同时诱导了小鼠的焦虑抑郁样行为。该研究证明了 IL-1β 基因敲除会对小鼠的基本认知行为和情绪行为造成损害, 为后续研究生理条件下炎症因子的正常表达对脑功能的作用提供了重要依据。

Interleukin-1β(IL-1β) is a common pro-inflammatory factor in the brain that is released by microglia and neurons at the physiological level and maintained at a low expression level. In this study, the effects of IL-1β gene knockout on the behavior of adult mice were investigated by using different behavioral paradigms. The results found that IL-1β gene knockout did not affect the basic motor ability of the mice, but significantly reduced the time and the frequency of the mice to enter the new arm of the Y maze and sniff new objects in the new object recognition test, and increased the immobility time of the mice in the tail suspension and forced swimming tests. These results indicated that the knockout of IL-1β gene reduced the learning and memory ability of mice and induced anxiety-like and depression-like behaviors in mice. The study proved that IL-1β gene knockout would damage the basic cognitive behavior and emotional behavior of mice, and provided an important basis for subsequent research on the effect of normal expression of inflammatory factors on brain function under physiological conditions.