丙二酸盐克罗诺杆菌 rpoS 基因突变株与回补株的构建

丙二酸盐克罗诺杆菌(Cronobacter malonaticus)在面对环境胁迫时,通过自身调节机制适应压力。rpoS基因作为一种稳定期σ因子和压力应答过程中的主要调节因子,在细菌抵抗或耐受环境胁迫压力中发挥重要作用。文章运用red同源重组技术和质粒表达系统,构建C.malonaticus G362的rpoS基因缺失株ΔrpoS和回补菌株ΔrpoS-com,为研究丙二酸盐克罗诺杆菌中rpoS基因的功能提供遗传材料。生长曲线测定结果表明,rpoS基因缺失未对正常培养条件下细菌生长产生显著影响。

Cronobacter malonaticus adapts to stress through self-regulation mechanism in the face of environmental stress. As a stable phase $ \sigma $ factor and a major regulator in the process of stress response, rpoS gene plays an important role in bacterial resistance or tolerance to environmental stress. In this paper, red homologous recombination technology and plasmid expression system were used to construct the rpoS gene deletion strain $ \Delta rpoS $ and complement strain $ \Delta rpoS $-com of C. malonaticus G362, providing genetic materials for studying the function of rpoS gene in C. malonaticus. The results of growth curve measurement showed that rpoS gene deletion had no significant effect on bacterial growth under normal culture conditions.