6 种饮食黄酮稳定肥大细胞活性的作用和机制研究

文章旨在探究白杨素、芹菜素、木犀草素、山奈酚、槲皮素和杨梅素稳定肥大细胞的生物活性及其机制。10、20、40 $ \mu $mol/L 6种饮食黄酮预处理骨髓来源肥大细胞（bone marrow derived mast cells，BMMCs），并用50 $ \mu $g/mL化合物48/80（C48/80）刺激细胞活化，采用酶联免疫吸附法和实时荧光定量聚合酶链式反应（polymerase chain reaction，PCR）检测细胞因子白介素6（interleukin 6，IL-6）、肿瘤坏死因子 $ \alpha $（tumor necrosis factor- $ \alpha $，TNF- $ \alpha $）的分泌量和mRNA表达水平，采用western blot检测细胞内丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）、p-MAPK、c-Jun氨基末端激酶（c-Jun N-terminal kinase，JNK）和p-JNK蛋白水平。结果表明，6种饮食黄酮对C48/80诱导的BMMCs活化具有剂量依赖的稳定作用，其中木犀草素和杨梅素的作用效果最强；机制研究显示木犀草素、槲皮素和杨梅素可通过下调MAPK和JNK的磷酸化水平抑制C48/80诱导的BMMCs活化。

This study aimed to explore the bioactivity and mechanism of mast cells stabilized by chrysin, apigenin, luteolin, kaempferol, quercetin, and myricetin. Bone marrow derived mast cells (BMMCs) were activated by $ 50 \mu g/mL $ compound 48/80(C48/80) after pretreatment with six dietary flavonoids at 10, 20, or $ 40 \mu mol/L $. The secretion and mRNA expression levels of interleukin 6(IL-6) and tumor necrosis factor- $ \alpha $(TNF- $ \alpha $) were detected by enzyme-linked immunosorbent assay (ELISA) and real-time fluorescent quantitative polymerase chain reaction (PCR). Western blot was used to detect the levels of mitogen-activated protein kinase (MAPK), p-MAPK, c-Jun N-terminal kinase (JNK) and p-JNK proteins in BMMCs. Results showed that six dietary flavonoids stabilized C48/80-induced BMMCs activation in a dose-dependent manner, of which luteolin and myricetin had the strongest effect. Mechanism studies showed that luteolin, quercetin, and myricetin inhibited C48/80-induced BMMCs activation by down-regulating the phosphorylation of MAPK and JNK.