大球盖菇多糖的体外模拟消化研究

文章从大球盖菇子实体中提取分离得到均一组分多糖(SP-1a)，采用体外模拟唾液、胃液和肠液模型，并结合理化性质与体外抗氧化活性，研究SP-1a在体外模拟消化体系中的变化。结果表明：唾液对SP-1a无显著影响；而在模拟胃液和肠液中，随着还原糖质量浓度的不断增加，SP-1a的分子量逐渐减小，其表面形态由大块片状变为网状聚集体，并且在高温下显现出不同的热解反应。此外体外抗氧化实验结果表明，SP-1a胃消化产物对·OH具有较强的清除能力，肠消化产物对DDPH自由基具有较强的清除能力及还原力。因此，SP-1a可在模拟胃肠体系中被消化降解，且其胃肠消化产物具有较强的抗氧化活性。

A homopolysaccharide(SP-1a) was extracted from the fruit bodies of Stropharia rugosoannulata. By simulating the digestion of human gastrointestinal tract in vitro and combining physicochemical properties with in vitro antioxidant activity, the changes of SP-1a in the simulated digestive system were explored. The results showed that saliva had no significant effect on SP-1a, but in gastrointestinal digestive system, with the increasing content of reducing sugars, the molecular weight of SP-1a gradually decreased, the surface morphology of SP-1a changed from large flakes to reticular aggregates, and it showed different pyrolysis reactions at high temperature. The results of in vitro antioxidant experiments showed that SP-1a gastric digestive products had strong $ \cdot $OH scavenging ability, and intestinal digestive products had strong DPPH scavenging ability and reducing power. Therefore, SP-1a can be digested in the simulated gastrointestinal system, and its gastrointestinal digestive products have strong antioxidant activity.