一种新的粒毛盘菌多糖对小鼠急性肝损伤的保护作用

为了研究一种新的粒毛盘菌 YM38 多糖 (LEP-1a) 对顺铂诱导急性肝损伤的保护作用,文章将 ICR 小鼠随机分为正常组、模型组、阳性组、LEP-1a 低剂量组 (100 mg/kg)、LEP-1a 高剂量组 (200 mg/kg) 共 5 组,每组 6 只。给予顺铂 (45 mg/kg) 建立急性肝损伤模型,测定小鼠各项生理指标。结果表明:与模型组相比,LEP-1a 能够显著降低小鼠肝指数以及肝组织中甘油三酯 (TG)、总胆固醇 (TC)、谷丙转氨酶 (ALT)、谷草转氨酶 (AST)、碱性磷酸酶 (AKP)、丙二醛 (MDA) 和过氧化氢酶 (CAT) 的水平,增强超氧化物歧化酶 (SOD) 的活性;抑制血清中炎性因子肿瘤坏死因子- $ \alpha $ (TNF- $ \alpha $) 和白细胞介素- $ \beta $ (IL-1 $ \beta $) 的表达水平,减轻肝组织中炎性细胞浸润。因此,LEP-1a 通过抑制氧化应激和炎症反应从而对顺铂诱导的急性肝损伤起到保护作用。

To study the protective effect of a novel polysaccharide from Lachnum YM38(LEP-1a) on acute liver injury induced by cisplatin, the ICR mice were randomly divided into five groups: normal group, model group, positive group, LEP-1a low dose group (100 mg/kg), LEP-1a high dose group (200 mg/kg) with six mice in each group. Cisplatin (45 mg/kg) was given to establish an acute liver injury model, and then various physiological indicators of the mice were measured. Results suggested that LEP-1a significantly reduced liver index and the hepatic contents of TG, TC, ALT, AST, AKP, MDA and CAT, and enhanced the SOD activity in mice when compared with the model group. At the same time, it inhibited the expression of inflammatory factors TNF- $ \alpha $ and IL-1 $ \beta $ in serum and reduced inflammatory cell infiltration in liver tissue. Therefore, LEP-1a can alleviate cisplatin-induced acute liver injury through inhibiting oxidative stress and inflammation.