菌-藻体系中无机碳源对小球衣藻生长和聚集的影响研究

为了提高微藻生长速率、实现低成本高效收集,文章以小球衣藻(Chlamydomonas microsphaera)为研究对象,探究无机碳源对菌-藻体系中微藻生长和聚集的影响。结果表明:无机碳源能够显著提高菌-藻体系中微藻生物量,当初始菌藻数量比为15:1时,在低碳源(37.6 mg/L)水平下,经过6 d培养后微藻生物量可达 $ 1.255 \times 10^{6} $个/mL,较纯藻培养体系增加30%;当碳源质量浓度升高至188.0 mg/L时,微藻数量较纯藻培养体系增加68%,可达 $ 1.681 \times 10^{6} $个/mL;菌-藻体系也有助于微藻自聚集,随着无机碳源质量浓度增加,其聚集效果变得更明显,聚集率最高可达66%,远高于纯培养体系中的27%。研究结果可为微藻综合利用研究提供参考。

In order to improve the growth rate of microalgae and achieve low-cost and high-efficiency collection, this paper takes Chlamydomonas microsphaera as the research object to explore the effects of inorganic carbon sources on the growth and aggregation of microalgae in the bacterial-algal system. The results showed that inorganic carbon sources could significantly increase the biomass of microalgae in the bacterial-algal system. When the initial ratio of bacteria to algae was 15:1, the biomass of microalgae could reach $ 1.255 \times 10^{6} $ cells/mL after six days of culture under the low carbon source (37.6 mg/L), which was 30% higher than that in the pure algal culture system. When the carbon source mass concentration increased to 188.0 mg/L, the number of microalgae increased by 68% compared with that of pure algal culture system, reaching $ 1.681 \times 10^{6} $ cells/mL. The bacterial-algal system also contributed to the self-aggregation of microalgae. With the increase of the mass concentration of inorganic carbon source, the aggregation effect became more obvious, and the aggregation rate was up to 66%, which was much higher than 27% in the pure culture system. The results can provide reference for the comprehensive utilization of microalgae.