炮制对多花黄精水提物抗氧化活性的影响

多花黄精（Polygonatum cyrtonema Hua）为百合科（Liliaceae）黄精属（Polygonatum Mill.）植物，是一种常见的“药食同源”植物，可经黄酒“九蒸九制”炮制。文章首先将炮制前后的多花黄精粉末经热水浸提获得炮制前后的水提物，经醇沉，取上清液再经离心、浓缩、干燥得到脂溶性部位，取醇沉物经除蛋白、透析、干燥以得到多糖部位，发现2个部位提取率存在明显差异；然后体外评价水提物的抗氧化活性，发现“九蒸九制”后水提物总抗氧化能力（total antioxidant capacity，T-AOC）和DPPH自由基清除能力均得到显著性提高；通过测定T-AOC和DPPH自由基清除能力等指标，发现炮制后水提物脂溶性部位和多糖部位体外抗氧化活性均显著高于炮制前。该文对了解多花黄精的药效学基础、临床应用和开发有效药物具有一定的意义。

Polygonatum cyrtonema Hua, a common medicinal and edible homologous plant, belongs to the genus Polygonatum Mill. (Liliaceae), which can be processed by nine steaming and nine processing with rice wine. In this paper, firstly, the aqueous extracts of crude and wine-processed P. cyrtonema Hua were obtained by hot water extraction. After that, the aqueous extracts were precipitated by alcohol, and the supernatants were centrifuged, concentrated and dried to obtain the liposoluble part. The alcohol precipitates were then deproteinized, dialysed and dried to obtain the polysaccharide part. And significant difference was found in extraction rate between the two parts. Then the antioxidant activity of the aqueous extracts was evaluated in vitro, the total antioxidant capacity(T-AOC) and free radical scavenging ability of DPPH of the aqueous extract were significantly increased after nine steaming and nine processing. By measuring the indexes of T-AOC and DPPH, it was found that the antioxidant activity of the liposoluble and polysaccharide parts of wine-processed P. cyrtonema Hua aqueous extract was significantly higher than that of crude P. cyrtonema Hua aqueous extract. This paper is of significance to understand the pharmacodynamic basis and clinical application of P. cyrtonema Hua.